HPLC法同时测定附子理中丸(浓缩丸)中甘草苷和甘草酸的含量
x

请在关注微信后,向客服人员索取文件

篇名: HPLC法同时测定附子理中丸(浓缩丸)中甘草苷和甘草酸的含量
TITLE:
摘要: 目的:建立同时测定附子理中丸(浓缩丸)中甘草苷和甘草酸含量的方法。方法:采用高效液相色谱法。色谱柱为WondaSil C18, 流动相为乙腈-0.05%磷酸溶液(梯度洗脱),流速为1.0 mL/min,检测波长为237 nm,柱温为35 ℃,进样量为10 μL。结果:甘草苷和甘草酸检测质量浓度线性范围分别为9.68~96.8 μg/mL(r=0.999 1)、14.08~140.8 μg/mL(r=0.999 2);定量限分别为0.2、0.3 μg/mL,检测限分别为0.1、0.01 μg/mL;精密度、稳定性、重复性试验的RSD<2.0%;加样回收率分别为98.9%~101.2%(RSD=0.62%,n=9)、98.6%~101.5%(RSD=1.06%,n=9)。结论:该方法操作简便、结果准确,可用于附子理中丸(浓缩丸)中甘草苷和甘草酸含量的同时测定。
ABSTRACT: OBJECTIVE: To establish the method for simultaneous determination of liquiritin and glycyrrhizic acid in Fuzi lizhong pills (condensed pills). METHODS: HPLC method was adopted. The determination was performed on WondaSil C18 column with mobile phase consisted of acetonitrile-0.05% phosphate solution (gradient elution) at the flow rate of 1.0 mL/min. The detection wavelength was set at 237 nm, and column temperature was 35 ℃. The sample size was 10 μL. RESULTS: The linear range of liquiritin and glycyrrhizic acid were 9.68-96.8 μg/mL(r=0.999 1)and 14.08-140.8 μg/mL(r=0.999 2). The limits of quantitation were 0.2, 0.3 μg/mL, and the limits of detection were 0.1, 0.01 μg/mL. RSDs of precision, stability and reproducibility tests were all lower than 2.0%. The average recoveries were 98.9%-101.2%(RSD=0.62%,n=9),98.6%-101.5%(RSD=1.06%,n=9). CONCLUSIONS: The method is simple and accurate, and can be used for simultaneous determination of liquiritin and glycyrrhizic acid in Fuzi lizhong pills (condensed pills).
期刊: 2017年第28卷第36期
作者: 方东伟,彭佳庆
AUTHORS: FANG Dongwei,PENG Jiaqing
关键字: 附子理中丸(浓缩丸);高效液相色谱法;甘草苷;甘草酸;含量测定
KEYWORDS: Fuzi lizhong pills (condensed pills); HPLC; Liquiritin; Glycyrrhizic acid; Content determination
阅读数: 375 次
本月下载数: 10 次

* 注:未经本站明确许可,任何网站不得非法盗链资源下载连接及抄袭本站原创内容资源!在此感谢您的支持与合作!