葡萄籽原花青素对肾缺血再灌注损伤大鼠肾组织中Caspase-3和TRAF6表达的影响
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篇名: 葡萄籽原花青素对肾缺血再灌注损伤大鼠肾组织中Caspase-3和TRAF6表达的影响
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摘要: 目的:研究葡萄籽原花青素对肾缺血再灌注损伤(RIRI)大鼠肾组织中半胱氨酸天冬氨酸蛋白酶3(Caspase-3) 和肿瘤坏死因子相关受体(TRAF6)表达的影响,探讨葡萄籽原花青素对RIRI的保护机制。方法:将50只大鼠随机分为假手术组、模型组、肾复康胶囊组(阳性对照,600 mg/kg)和葡萄籽原花青素低、高剂量组(100、150 mg/kg),每组10只。每天ig给药1次,连续7 d。给药结束后,除假手术组的其余各组大鼠均复制RIRI模型。造模成功24 h后,检测各组大鼠血清中肌酐(Cr)、尿素氮(BUN)水平以及肾组织中Caspase-3、 TRAF6蛋白表达,并测定肾小管上皮细胞凋亡率。结果:与假手术组比较,模型组大鼠血清中Cr、BUN水平明显升高(P<0.05),肾组织中Caspase-3、TRAF6蛋白表达明显增强(P<0.05),肾小管上皮细胞凋亡率明显升高(P<0.05);与模型组比较,各给药组大鼠血清中Cr、BUN水平均明显降低(P<0.05),肾组织中Caspase-3、TRAF6蛋白表达均明显减弱(P<0.05),肾小管上皮细胞凋亡率均明显降低(P<0.05),且葡萄籽原花青素高剂量组作用优于葡萄籽原花青素低剂量组和肾复康胶囊组(P<0.05)。结论:葡萄籽原花青素能减轻大鼠RIRI,这可能是通过下调肾组织中Caspase-3、TRAF6蛋白的表达,从而抑制肾小管上皮细胞凋亡来实现的。
ABSTRACT: OBJECTIVE: To study the effect of grape seed procyanidins on protein expressions of cysteine aspartic protease 3 (Caspase-3), tumor necrosis factor-related receptors (TRAF6) in renal tissue of rats with renal ischemia-reperfusion injury (RIRI), and explore the protective mechanism of procyanidins on RIRI. METHODS: 50 rats were randomly divided into sham operation group, model group, Shenfukang capsules group (positive control, 600 mg/kg), procyanidins low-dose, high-dose groups (100, 150 mg/kg), 10 in each group. All rats were intragastrically administrated once a day, for 7 d. After administration,rats in other groups except for sham operation group were established the RIRI model.After the modeling successed of 24 h,levels of creatinine (Cr), urea nitrogen (BUN) in serum, and protein expressions of Caspase-3, TRAF6 in renal tissue of rats in each group were detected, and apoptotic rate of renal tubular epithelial cells was determined. RESULTS: Compared with sham operation group, levels of Cr, BUN in serum in model group were significantly increased (P<0.05); protein expressions of Caspase-3, TRAF6 in renal tissue were obviously enhanced (P<0.05); apoptotic rate of renal tubular epithelial cells was obviously increased (P<0.05). Compared with model group, levels of Cr, BUN in serum in each administration group were obviously decreased (P<0.05); protein expressions of Caspase-3, TRAF6 in renal tissue were obviously weakened (P<0.05); apoptotic rate of renal tubular epithelial cells was obviously decreased (P<0.05); and procyanidins high-dose group showed superior effect to low-dose group and Shenfukang granules group (P<0.05). CONCLUSIONS: Grape seed procyanidins can relieve the RIRI of rats, which may be achieved by reducing protein expressions of Caspase-3 and TRAF6 to inhibit the apoptotic rate of renal tubular epithelial cells.
期刊: 2017年第28卷第34期
作者: 宋云梅
AUTHORS: SONG Yunmei
关键字: 葡萄籽原花青素;肾缺血再灌注损伤;半胱氨酸天冬氨酸蛋白酶3;肿瘤坏死因子相关受体;大鼠
KEYWORDS: Grape seed procyanidins; Renal ischemia-reperfusion injury; Cysteine aspartic protease 3; Tumor necrosis factor-related receptors; Rats
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