当归芍药散对围绝经期模型大鼠子宫结构及雌激素受体表达的影响
x

请在关注微信后,向客服人员索取文件

篇名: 当归芍药散对围绝经期模型大鼠子宫结构及雌激素受体表达的影响
TITLE:
摘要: 目的:探讨当归芍药散(DSS)对围绝经期模型大鼠子宫结构及子宫腔上皮和基质中雌激素受体α(ERα)、雌激素受体β(ERβ)表达的影响。方法:将40只雌性SD大鼠随机分为假手术组(生理盐水)、模型组(生理盐水)和DSS低、中、高剂量组(1.94、3.87、7.44 g/kg),每组8只。除假手术组大鼠切除卵巢附近脂肪外,其余各组大鼠切除双侧卵巢以建立围绝经期模型。造模成功后,大鼠每天ig给药1次,连续8周。给药结束后,称定大鼠子宫湿质量,观察大鼠子宫形态和结构的变化,测定子宫腔上皮及基质中ERα、ERβ表达水平。结果:与假手术组比较,模型组大鼠子宫内膜柱状上皮呈低柱状,固有层、肌层与浆膜层均显著萎缩,基质细胞可见明显核固缩,子宫湿质量、宫腔面积、内膜厚度及腺体数量均显著减少(P<0.01),子宫腔上皮及基质中ERα、ERβ表达水平均显著降低(P<0.01)。与模型组比较,DSS各剂量组大鼠子宫内膜及固有层萎缩程度差异无统计学意义,但固有层内腺体丰富,子宫湿质量、宫腔面积、内膜厚度以及子宫腔上皮和基质中ERα表达水平差异均无统计学意义(P>0.05),但DSS中、高剂量组大鼠子宫腺体数量显著增加(P<0.01),DSS高剂量组大鼠子宫腔上皮及基质中ERβ表达水平显著升高(P<0.05或P<0.01)。结论:DSS对围绝经期模型大鼠子宫腺体萎缩症状的改善作用不明显,但可增加模型大鼠的腺体数量,这可能与提高子宫腔上皮及基质中ERβ表达水平有关。
ABSTRACT:

OBJECTIVE: To investigate the effect of Danggui shaoyao powder (DSS) on uterine structure and expressions of estrogen receptor α (ERα), estrogen receptor β (ERβ) in uterine cavity epithelium and matrix in model rats in perimenopausal period. METHODS: 40 female SD rats were randomly divided into sham operation group (normal saline), model group (normal saline), DSS low-dose, medium-dose, high-dose groups (1.94, 3.87, 7.44 g/kg), 8 in each group. Except that rats in sham operation group received resection of fat nearby ovaries, rats in other groups received resection of bilateral ovaries to induce models in perimenopausal period. After modeling, rats were intragastrically administrated once a day, for 8 weeks. After administration, wet mass of uterine was weighted. Changes in uterine morphology and structure of rats were observed, expressions levels of ERα and ERβ in uterine cavity epithelium and matrix were determined. RESULTS: Compared with sham operation group, rats in model group showed low columnar in endometrial columnar epithelium, lamina propria layer, muscular layer and serous layer were significantly atrophied, stromal cells had obvious nuclear condensation. There was marked decrease in uterine wet mass, uterine cavity area and endometrial thickness as well as the number of uterine glands (P<0.01), and the expression levels of ERα and ERβ in uterine cavity epithelium and matrix were significantly reduced (P<0.01). Compared with model group, the atrophy degree of endometrium and lamina propria layer had no significant differences in DSS each dose groups. However, lamina propria layer was rich in glands, and there were significant differences in uterine wet mass, uterine cavity area, endometrial thickness, ERα expression level in uterine cavity epithelium and matrix (P>0.05). However, the number of uterine glands in DSS medium-dose, high-dose groups was significantly increased (P<0.01), and ERβ expression level in uterine cavity epithelium and matrix in DSS high-dose group was significantly increased (P<0.05 or P<0.01). CONCLUSIONS: DSS has not obvious effect on improving the symptoms of uterine gland atrophy of model rats in perimenopausal period, but it can increase the number of uterine glands,and the mechanism may be associated with improving the ERβ expression level in uterine cavity epithelium and matrix.


期刊: 2017年第28卷第34期
作者: 丁岩,华永庆,林紫微,杨菲,洪敏
AUTHORS: DING Yan,HUA Yongqing,LIN Ziwei,YANG Fei,HONG Min
关键字: 当归芍药散;围绝经期;子宫结构;雌激素受体α;雌激素受体β;大鼠
KEYWORDS: Danggui shaoyao powder; Perimenopausal period; Uterine structure; Estrogen receptor α; Estrogen receptor β; Rats
阅读数: 230 次
本月下载数: 2 次

* 注:未经本站明确许可,任何网站不得非法盗链资源下载连接及抄袭本站原创内容资源!在此感谢您的支持与合作!