温阳振衰颗粒对慢性心衰模型大鼠心肌中MEF2表达的影响
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篇名: 温阳振衰颗粒对慢性心衰模型大鼠心肌中MEF2表达的影响
TITLE:
摘要: 目的:研究温阳振衰颗粒对慢性心衰(CHF)模型大鼠心肌中肌细胞增强子(MEF2)表达的影响。方法:将60只大鼠随机抽取10只作为正常对照组(生理盐水),其余50只大鼠以阿霉素建立CHF模型后随机分为模型对照组(生理盐水)、阳性对照组[依那普利1.8 mg/(kg·d)]和温阳振衰颗粒低、中、高剂量组[0.72、1.44、2.88 g/(kg·d)],每天ig给药2次,连续给药4周。给药结束后,检测各组大鼠心功能指标[左心室射血分数(LVEF)、左心室短轴缩短率(LVFS)、左心室收缩末期内径(LVSD)和左心室舒张末期内径(LVDD)],检测各组大鼠心肌中MEF2和磷酸化MEF2(p-MEF2)mRNA及其蛋白的表达。结果:与正常对照组比较,模型对照组和各给药组大鼠LVEF、LVFS明显降低(P<0.05),LVSD、LVDD明显升高(P<0.05);心肌中p-MEF2蛋白表达明显下调(P<0.05),MEF2 mRNA及蛋白表达差异无统计学意义(P>0.05)。与模型对照组比较,各给药组大鼠心功能指标均明显改善(P<0.05);心肌中p-MEF2蛋白表达均明显上调(P<0.05),MEF2 mRNA及蛋白表达差异均无统计学意义(P>0.05)。结论:温阳振衰颗粒能拮抗阿霉素诱导的MEF2蛋白磷酸化的下调作用,这可能是其治疗CHF的机制之一。
ABSTRACT: OBJECTIVE: To study the effect of Wenyang zhenshuai granules on myocardial MEF2 in model rats with chronic heart failure (CHF). METHODS: 10 rats were randomly selected in 60 rats as normal control group (normal saline), the remaining 50 rats were randomly divided into model control group (normal saline), positive control group [enalapril, 1.8 mg/(kg·d)], Wenyang zhenshuai granules low-dose, medium-dose, high-dose groups [0.72, 1.44, 2.88 g/(kg·d)] after induced to CHF models by adriamycin, intragastrically administrated twice a day, for 4 weeks. After administration, heart function indexes [left ventricular ejection fraction (LVEF), left ventricular fractional shortening (LVFS), left ventricular end-diastolic diameter (LVSD) and left ventricular end-diastolic diameter (LVDD)] of rats in each group were detected, protein expressions of myocardial MEF2 and phosphorylated MEF2 (p-MEF2) and mRNA expression of MEF2 were determined. RESULTS: Compared with normal control group, LVEF, LVFS of rats in model control group and each administration group were significantly reduced (P<0.05), LVSD, LVDD were significantly increased (P<0.05); protein expression of myocardial p-MEF2 was significantly down-regulated (P<0.05), and there were no statistical significances in protein or mRNA expressions of MEF2 (P>0.05). Compared with model control group, heart function indexes of rats in each administration group were significantly improved (P<0.05);protein expression of myocardial p-MEF2 was significantly up-regulated (P<0.05), and there were no statistical significances in protein or mRNA expressions of MEF2 (P>0.05). CONCLUSIONS: Wenyang zhenshuai granule can antagonize the down-regulation effect of adriamycin-induced MEF2 protein phosphorylation, which may be one of the mechanisms of its treatment for CHF.
期刊: 2017年第28卷第34期
作者: 蔡虎志,王笑莹,陈青扬,陈新宇
AUTHORS: CAI Huzhi,WANG Xiaoying,CHEN Qingyang,CHEN Xinyu
关键字: 温阳振衰颗粒;慢性心衰; 心肌细胞增强子;磷酸化心肌细胞增强子;大鼠
KEYWORDS: Wenyang zhenshuai granules; Chronic heart failure; Myocardial cell enhancer; Phosphorylated myocardial cell enhancer; Rats
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