了哥王药材中7种成分的含量测定及其主成分、聚类分析
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篇名: 了哥王药材中7种成分的含量测定及其主成分、聚类分析
TITLE:
摘要: 目的:建立同时测定了哥王药材中7种成分含量的方法,同时对其进行主成分分析及聚类分析。方法:采用高效液相色谱法。色谱柱为Diamonsil Platisil ODS,流动相为乙腈-0.15%三乙胺溶液(磷酸调pH至 6.0)(梯度洗脱),流速为1.0 mL/min,检测波长为280 nm,柱温为40 ℃,进样量为10 μL。采用SPSS 22.0统计软件对含量测定结果进行主成分分析与聚类分析。结果:柚皮苷、杨梅素、牛蒡苷、木犀草素、槲皮素、芹菜素和西瑞香素检测质量浓度线性范围分别为2.688~53.76 μg/mL(r=0.999 8)、5.052~101.00 μg/mL(r=0.999 9)、2.052~41.04 μg/mL(r=0.999 9)、2.108~42.16 μg/mL(r=0.999 9)、5.112~102.20 μg/mL(r=0.999 9)、0.820~16.42 μg/mL(r=0.999 9)、2.070~41.40 μg/mL(r=0.999 9);定量限≤1.072 0 μg/mL,检测限≤0.331 8 μg/mL;精密度、稳定性、重复性试验的RSD<2.0%;加样回收率分别为97.8%~102.5%(RSD=1.8%,n=6)、97.2%~102.0%(RSD=2.0%,n=6)、95.2%~100.1%(RSD=1.7%,n=6)、95.2%~99.3%(RSD=1.6%,n=6)、97.0%~100.8%(RSD=1.3%,n=6)、95.5%~98.6%(RSD=1.1%,n=6)、95.0%~99.3%(RSD=1.8%,n=6)。10批药材样品有3个主成分。10个产地的药材样品可聚为2类;广东清远和贵州贵阳产地的药材样品质量较好。结论:该方法操作简便,精密度、稳定性、重复性好,可用于了哥王药材中7种成分含量的同时测定;不同产地的了哥王药材质量差异较大。
ABSTRACT: OBJECTIVE: To establish a method for simultaneous determination of 7 constituents in Wikstroemia indica, and to conduct principal component analysis and cluster analysis. METHODS: HPLC method was adopted. The determination was performed on Diamonsil Platisil ODS column with mobile phase consisted of acetonitrile-0.15% triethylamine solution (pH adjusted to 6.0 with phosphoric acid, gradient elution) at the flow rate of 1.0 mL/min. The detection wavelength was set at 280 nm, and column temperature was 40 ℃. The sample size was 10 μL. The principal component analysis and cluster analysis were conducted for the results of content determination by SPSS 22.0 statistical software. RESULTS: The linear ranges were 2.688-53.76 μg/mL for naringin (r=0.999 8), 5.052-101.00 μg/mL for myricetin (r=0.999 9), 2.052-41.04 μg/mL for arctiin (r=0.999 9), 2.108-42.16 μg/mL(r=0.999 9), 5.112-102.20 μg/mL (r=0.999 9), 0.820-16.42 μg/mL (r=0.999 9), 2.070-41.40 μg/mL(r=0.999 9), respectively. The limits of quantitation were no higher than 1.072 0 μg/mL, the limits of detection were no higher than 0.331 8 μg/mL. RSDs of precision, stability and reproducibility tests were all lower than 2.0%. The recoveries were 97.8%-102.5%(RSD=1.8%,n=6),97.2%-102.0%(RSD=2.0%,n=6),95.2%-100.1%(RSD=1.7%,n=6),95.2%-99.3%(RSD=1.6%,n=6), 97.0%-100.8%(RSD=1.3%,n=6), 95.5%-98.6%(RSD=1.1%,n=6), 95.0%-99.3%(RSD=1.8%,n=6), respectively. Three main components were belong to the samples of 10 batches of medicinal materials. The samples of medicinal materials from 10 producing area could be divided into 2 categories. The quality of W. indica from Qingyuan Guangdong and Guiyang Guizhou were better than others.  CONCLUSIONS: The method is simple, precise, stable and reproducible, and it can be used for simultaneous determination of 7 constituents in medicinal material. The quality of W. indica from different regions are quite different.
期刊: 2017年第28卷第33期
作者: 荚丽丽,魏岚,赵健,贾玉迪,邱雨,杜超颖,孙立新
AUTHORS: JIA Lili,WEI Lan,ZHAO Jian,JIA Yudi,QIU Yu,DU Chaoying,SUN Lixin
关键字: 了哥王;高效液相色谱法;含量测定;主成分分析;聚类分析
KEYWORDS: Wikstroemia indica; HPLC; Content determination; Principal component analysis; Cluster analysis
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