藏药水柏枝的质量标准研究
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篇名: 藏药水柏枝的质量标准研究
TITLE:
摘要: 目的:建立藏药水柏枝的质量标准。方法:从性状特征、显微特征、薄层色谱(TLC)方面对药材进行定性鉴别;检测药材水分、灰分和浸出物的含量;采用高效液相色谱法(HPLC)测定药材中没食子酸的含量:色谱柱为Diamonsil C18,流动相为乙腈-0.1%磷酸溶液(5 ∶ 95,V/V),流速为1.0 mL/min,检测波长为270 nm,柱温为30 ℃,进样量为10 μL。结果:水柏枝呈圆柱形,有多数窄条形互生小叶,质脆,易折断,气微,味淡。木栓细胞表面和下表皮细胞表面可见多角形,木纤维多成束,草酸钙簇晶众多。TLC图斑点清晰,分离度良好。水分为6.83%~8.12%,总灰分为4.01%~5.01%,酸不溶性灰分为1.06%~1.98%,水浸出物为17.91%~22.65%,醇浸出物为11.29%~15.51%。没食子酸检测质量浓度线性范围为3.13~50 μg/mL(r=0.999 7),精密度、稳定性、重复试验的RSD<1.0%,没食子酸加样回收率为94.0%~100.8%(RSD=2.27%,n=9)。结论:该研究所建标准可用于藏药水柏枝的质量评价。
ABSTRACT: OBJECTIVE: To establish the quality standard of Tibetan medicine Myricaria germanica. METHODS: M. germanica was identified in respects of properties, microscopic characteristics and TLC. The contents of moisture, ash and extract were determined. HPLC method was adopted for content determination of gallic acid. The determination was performed on Diamonsil C18 column with mobile phase consisted of acetonitrile -0.1% phosphoric acid solution(5 ∶ 95,V/V) at the flow rate of 1.0 mL/min. The detection wavlengths were set at 270 nm. The column temperature was 30 ℃ and the sample size was 10 μL. RESULTS: M. germanica was cylindrical in shape, and there were many narrow strip alternate leaflets, which were brittle, easily broken, weak in smell, mild in flavor. Cork cell surface and lower epidermal cell surface showed polygon; most of xylon were bunched; there were many clusters of calcium oxalate. TLC spots were clear and well-separated. The contents of moisture, total ash, acid-insoluble ash, water-soluble extract and ethanol were 6.83%-8.12%,4.01%-5.01%,1.06%-1.98%,17.91%-22.65%, 11.29%-15.51%. The linear range of gallic acid were 3.13-50 μg/mL(r=0.999 7); RSDs of precision, stability and reproducibility tests were lower than 1.0%; the recoveries were 94.0%-100.8%(RSD=2.27%,n=9). CONCLUSIONS: Established standard can be used for quality evaluation of Tibetan medicine M. germanica.
期刊: 2017年第28卷第33期
作者: 多杰,马志良,多杰拉旦,尼玛才让,东知多杰,赛桑杰
AUTHORS: DUO Jie,MA Zhiliang,Duojieladan,Nimacairang,Dongzhiduojie,Saisangjie
关键字: 藏药;水柏枝;质量标准;没食子酸;薄层色谱法;高效液相色谱法
KEYWORDS: Tibetan medicine; Myricaria germanica; Quality standard; Gallic acid; TLC; HPLC
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