高效液相色谱法测定人血浆中伏立康唑的浓度
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篇名: 高效液相色谱法测定人血浆中伏立康唑的浓度
TITLE:
摘要: 目的:建立测定人血浆中伏立康唑浓度的方法。方法:血浆样品经乙腈沉淀蛋白后,以酮康唑为内标,采用高效液相色谱法测定。色谱柱为Dionex U-3000 Dimonsil C18,流动相为三乙胺-冰醋酸-水混合溶液(1 ∶ 1 ∶ 98,V/V/V,pH约为4.0)-乙腈(40 ∶ 60,V/V),流速为1.0 mL/min,检测波长为255 nm,柱温为40 ℃,进样量为20 μL。结果:伏立康唑血药浓度在0.2~20.0 μg/mL范围内线性关系良好,定量下限为0.2 μg/mL,最低检测限为0.03 μg/mL;日内、日间RSD均小于10%;方法回收率为92.06%~106.26%(RSD<5%,n=5),提取回收率为75.62%~90.59%(RSD<5%,n=5)。采用该法测得10例患儿体内伏立康唑的血药浓度为0.22~4.90 μg/mL(n=10)。结论:该方法简单、快速、专属性强,可用于伏立康唑治疗药物监测。
ABSTRACT: OBJECTIVE: To establish a method for the concentration determination of voriconazole in human plasma. METHODS: Plasma samples were precipitated with acetonitrile. Using ketoconazole as internal standard, HPLC method was adopted. The determination was performed on Dionex U-3000 Dimonsil C18 column with mobile phase consisted of triethylamine-glacial acetic acid-water mixed solution (1 ∶ 1 ∶ 98,V/V/V, pH was about 4.0)-acetonitrile (40 ∶ 60,V/V) at the flow rate of 1.0 mL/min. The detection wavelength was set at 255 nm. The column temperature was 40 ℃, and sample size was 20 μL. RESULTS: The linear range of voriconazole was 0.2-20.0 μg/mL. The limits of quantification was 0.2 μg/mL, and the minimum detection limit was 0.03 μg/mL. RSDs of inter-day and intra-day were lower than 10%. The method recoveries were 92.06%-106.26%(RSD<5%,n=5), and extraction recoveries were 75.62%-90.59%(RSD<5%,n=5). The plasma concentration of voriconazole in 10 children ranged 0.22-4.90 μg/mL (n=10). CONCLUSIONS: The method is simple, rapid, specific and can be used for drug monitoring of voriconazole.
期刊: 2017年第28卷第32期
作者: 李学娟,刘四喜,陈泽彬,魏红,杨卫国,温鹏强
AUTHORS: LI Xuejuan,LIU Sixi,CHEN Zebin,WEI Hong,YANG Weiguo,WEN Pengqiang
关键字: 伏立康唑;血药浓度;高效液相色谱法
KEYWORDS: Voriconazole; Plasma concentration; HPLC
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