水药金耳环多糖对实验性2型糖尿病模型大鼠糖脂代谢、肾功能及氧化应激的影响
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篇名: 水药金耳环多糖对实验性2型糖尿病模型大鼠糖脂代谢、肾功能及氧化应激的影响
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摘要: 目的:研究水药金耳环多糖对实验性2型糖尿病模型大鼠糖脂代谢、肾功能及氧化应激的影响,为金耳环多糖的开发利用提供参考。方法:将60只SD大鼠随机分为正常对照组、模型对照组、阳性对照组(厄贝沙坦片,0.02 g/kg)和金耳环多糖低、中、高剂量组(以生药计分别为2、4、8 g/kg),每组10只。除正常对照组外,其余各组大鼠均ip链脲佐菌素75 mg/(kg·d)复制糖尿病大鼠模型。成模后,各给药组大鼠ig相应药物,正常对照组和模型对照组大鼠ig等体积5%羧甲基纤维素钠溶液,每天2次,连续42 d。给药结束后,采用紫外分光光度计检测肝组织中肝糖原含量;采用全自动生化分析仪检测大鼠24 h尿量、24 h尿蛋白含量,血清中三酰甘油(TG)、胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)、肌酐(Cr)、尿素氮(BUN)水平,以及肾组织中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT)、超氧化物歧化酶(ROS)、丙二醛(MDA)水平。结果:与正常对照组比较,模型对照组大鼠肝组织中肝糖原含量减少(P<0.05);24 h尿量、24 h尿蛋白量增加(P<0.05);血清中TG、TC、LDL-C水平升高(P<0.05),HDL-C水平降低(P<0.05);肾组织中SOD、CAT、GSH-Px水平降低(P<0.05),ROS、MDA水平升高(P<0.05)。与模型组比较,除肝组织中肝糖原含量、血清中HDL-C水平以及金耳环多糖低剂量组大鼠24 h尿量改善不显著外,其余各给药组大鼠上述指标均得到明显改善(P<0.05)。结论:金耳环多糖可调节实验性2型糖尿病模型大鼠的血脂代谢紊乱、改善大鼠肾功能和提高肾抗氧化能力。
ABSTRACT: OBJECTIVE: To study the effect of Shui medicine Asarum insigne polysaccharide on glycolipid metabolism, renal function and oxidative stress in rats with experimental type 2 diabetes, and provide reference for its development and use. METHODS: 60 SD rats were randomly divided into normal control group, model control group, positive control group (Irbesartan tablets, 0.02 g/kg) and A. insigne polysaccharide low-dose, medium-dose, high-dose groups (calculated by crude drug as 2, 4, 8     g/kg), 10 in each group. Except for normal control group, rats in other groups were intraperitoneally injected streptozotocin 75   mg/(kg·d) to induce model with diabetes. After modeling, rats in each administration group were intraperitoneally injected relevant medicines, and rats in normal control group and model control group were intragastrically administrated 5% carboxymet hylcellulose sodium solution, twice a day, for 42 d. After administration, UV spectrophotometer was used to detect the glycogen content in liver tissue. Automatic biochemical analyzer was used to detect 24 h urine output, 24 h urinary protein content of rats, levels of triglyceride (TG), cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), creatinine (Cr), urea nitrogen (BUN) in serum, and levels of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT), superoxide dismutase (ROS), malondialdehyde (MDA) in kidney tissue. RESULTS: Compared with normal control group, glycogen content in liver tissue of rats in model control group was decreased (P<0.05); 24 h urine output and 24 h urinary protein content were increased (P<0.05); levels of TG, TC, LDL-C in serum were increased (P<0.05), and HDL-C level was decreased (P<0.05); levels of SOD, CAT, GSH-Px in kidney tissue were decreased (P<0.05), and levels of ROS, MDA were increased (P<0.05). Compared with model group, except that there were no significant differences in the improvement of urinary protein content, HDL-C level in serum, 24 h urine output in A. insigne polysaccharide low-dose group, above-mentioned indexes in each administration group were obviously improved (P<0.05). CONCLUSIONS: A. insigne polysaccharide can regulate lipid metabolic disorders, and improve renal function and antioxidant capacity of rats.
期刊: 2017年第28卷第31期
作者: 周多强,李溥,罗良琦,杨再波,王其丽
AUTHORS: ZHOU Duoqiang,LI Pu,LUO Liangqi,YANG Zaibo,WANG Qili
关键字: 水药;金耳环多糖;2型糖尿病;血糖;血脂;肾损伤;氧化应激;大鼠
KEYWORDS: Shui medicine; Asarum insigne polysaccharide; Type 2 diabetes; Blood glucose; Blood lipid; Renal injury; Oxidative stress; Rats
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