蒙药五味沙棘散对吸烟致小鼠肺部炎症的改善作用及机制研究
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篇名: 蒙药五味沙棘散对吸烟致小鼠肺部炎症的改善作用及机制研究
TITLE:
摘要: 目的:研究蒙药五味沙棘散( WSP)对吸烟所致小鼠肺部炎症的改善作用及其机制。方法:将30只ICR小鼠随机分为空白组(生理盐水)、模型组(生理盐水)和WSP组(2 g/kg)。模型组和WSP组小鼠采用被动吸烟法复制肺部炎症损伤模型,并于造模的同时每天ig相应药物1次,连续28 d。给药结束后,采用酶联免疫吸附法检测小鼠肺泡灌洗液(BALF)中肿瘤坏死因子α(TNF-α)、白细胞介素1β(IL-1β)、IL-6和IL-10水平;苏木精-伊红染色后光镜下观察小鼠肺组织病理变化;Western blot法检测小鼠肺组织中细胞外信号调节蛋白激酶1/2(ERK1/2)、磷酸化ERK1/2(p-ERK1/2)、p38丝裂原活化蛋白激酶(p38 MAPK)、磷酸化p38 MAPK(p-p38 MAPK)、核因子κB p65(NF-κB p65)和磷酸化NF-κB p65(p-NF-κB p65)蛋白表达。结果:与空白组比较,模型组小鼠BALF中TNF-α、IL-1β和IL-6水平明显升高(P<0.01),肺组织发生明显炎症病变,肺组织中p-ERK1/2、p-p38 MAPK、p-NF-κB p65蛋白表达水平明显升高(P<0.01)。与模型组比较,WSP组大鼠BALF中TNF-α、IL-1β水平明显降低(P<0.05),肺组织炎症损伤明显改善,肺组织中p-p38 MAPK和p-NF-κB p65蛋白表达水平明显降低(P<0.05)。结论:WSP可能通过阻断p38 MAPK、NF-κB p65蛋白的磷酸化来抑制炎症因子TNF-α和IL-6的高表达,从而发挥其对吸烟所致小鼠肺部炎症损伤的改善作用。
ABSTRACT: OBJECTIVE: To study the improvement effect and mechanism of Meng medicine Wuwei Shaji powder (WSP) on smoke-induced lung inflammation injury in mice. METHODS: ICR mice were randomly divided into blank group (normal saline), model group (normal saline) and WSP group (2 g/kg). Mice in model group and WSP group received passive smoking to induce model of lung inflammation injury, and intragastrically administrated relevant medicine when modeling, once a day, for 28 d. After administration, enzyme-linked immunosorbent assay was used to detect the tumor necrosis factor α (TNF-α), interleukin-1β (IL-1β), IL-6, IL-10 levels in bronchoalveolar lavage fluids (BALF); the pathological changes of lung tissue were observed by optical microscope after hematoxylin-eosin staining; Western blot was adopted to detect the protein expressions of extracellular signal-regulated kinase (ERK1/2), phosphorylated ERK1/2 (p-ERK1/2), p38 mitogen-activated protein kinase (p38 MAPK), phosphorylated p38 MAPK (p-p38 MAPK), nuclear factor kappaB p65 (NF-κB p65) and phosphorylated NF-κB p65 (p-NF-κB p65) in lung tissue of mice. RESULTS: Compared with blank group, TNF-α, IL-1β, IL-6 levels in BALF in model group were obviously increased (P<0.01); lung tissue showed significant inflammatory lesions; and the protein expressions of p-ERK1/2, p-p38 MAPK, p-NF-κB p65 in lung tissue were obviously increased (P<0.01). Compared with model group, TNF-α, IL-1β levels in BALF in WSP group were obviously decreased (P<0.05); inflammation injury in lung tissue was obviously improved; and protein expressions of p-p38 MAPK and p-NF-κB p65 in lung tissue were obviously decreased (P<0.05). CONCLUSIONS: WSP shows improvement effect on smoke-induced lung inflammation injury in mice, which might be by blocking the p38 MAPK, NF-κB p65 phosphorycation to inhibit the high expression of inflammatory factors as TNF-α and IL-6.
期刊: 2017年第28卷第31期
作者: 包桂兰,王秀枝,梁凤娟,刘佳,刘铮
AUTHORS: BAO Guilan,WANG Xiuzhi,LIANG Fengjuan,LIU Jia,LIU Zheng
关键字: 蒙药;五味沙棘散;吸烟;炎症;小鼠
KEYWORDS: Meng medicine; Wuwei Shaji powder; Smoke; Inflammation; Mice
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