人参皂苷CK联合5-氟尿嘧啶对人胰腺癌PANC-1细胞的增殖、凋亡及上皮间质转化的影响
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篇名: 人参皂苷CK联合5-氟尿嘧啶对人胰腺癌PANC-1细胞的增殖、凋亡及上皮间质转化的影响
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摘要: 目的:研究人参皂苷CK联合5-氟尿嘧啶(5-FU)对人胰腺癌PANC-1细胞的增殖、凋亡及上皮间质转化(EMT)的影响。方法:将对数生长期的PANC-1细胞分为空白对照组、人参皂苷CK组(30 mg/L)、5-FU组(25 mg/L)和联用组(人参皂苷CK 30 mg/L+5-FU 25 mg/L)。采用MTT法检测各组细胞作用24、48、72 h后的细胞增殖抑制率,流式细胞术检测作用48 h后的细胞凋亡率,酶联免疫吸附法检测作用24、48、72、96 h后细胞中纤连蛋白表达,Western blot法检测作用48 h后细胞中波形蛋白、N-钙黏蛋白、E-钙黏蛋白、蛋白激酶(Akt)及磷酸化Akt(p-Akt)蛋白表达。结果:人参皂苷CK、5-FU及其二者联用对细胞的增殖均有抑制作用;与空白对照组比较,人参皂苷CK组、5-FU组和联用组各作用时间点的早期和晚期凋亡率、E-钙黏蛋白表达水平均明显升高(P<0.05),纤连蛋白、波形蛋白、N-钙黏蛋白表达水平和p-Akt/Akt水平均明显降低(P<0.05),其中联用组上述指标效果均优于人参皂苷CK组和5-FU组(P<0.05)。结论:人参皂苷CK和5-FU均可抑制PANC-1细胞增殖、诱导细胞凋亡、抑制EMT,该作用可能与抑制磷脂酰肌醇3-激酶/Akt通路有关;且二者联用效果更好。
ABSTRACT: OBJECTIVE: To study the effects of ginsenoside CK combined with 5-fluorouracil (5-FU) on the proliferation, apoptosis and epithelial mesenchymal transition (EMT) of human pancreatic cancer PANC-1 cells. METHODS: PANC-1 cells of logarithmic growth phase were randomly divided into blank control group, ginsenoside CK group (30 mg/L), 5-FU group (25   mg/L) and combination group (ginsenoside CK 30 mg/L+5-FU 25 mg/L). MTT method was used to detect the cell proliferation inhibition rate in each group after 24, 48, 72 h; flow cytometry was used to detect the cell apoptosis rate after 48 h; enzyme-linked immunosorbent assay was used to detect the fibronectin expression in cells after 24, 48, 72, 96 h; and Western blot was used to detect the expressions of vimentin, N-cadherin, E-cadherin, protein kinase (Akt) and phosphorylated Akt (p-Akt) protein in cells after 48 h. RESULTS: Compared with blank control group, the cell proliferation inhibition rate, early and late apoptotic rates, protein expression level of E-cadherin in ginsenoside CK group, 5-FU group and combination group were obviously increased (P<0.05), while the protein expression levels of fibronectin, vimentin, N-cadherin, and p-Akt/Akt levels were obviously decreased (P<0.05); the effects of above-mentioned indexes in combination group were superior to ginsenoside CK group and 5-FU group (P<0.05). CONCLUSIONS: Both ginsenoside CK and 5-FU can inhibit the proliferation of PANC-1 cells, induce their apoptosis and inhibit EMT, which may be associated with inhibiting phosphatidylinositol 3-kinase/Akt pathway. In addition, the combination of ginsenoside CK and 5-FU can produce a better effect.
期刊: 2017年第28卷第31期
作者: 崔江河,韩光宇,何光梅,尹媛媛,马娜,刘琳
AUTHORS: CUI Jianghe,HAN Guangyu,HE Guangmei,YIN Yuanyuan,MA Na,LIU Lin
关键字: 人参皂苷CK;5-氟尿嘧啶;人胰腺癌PANC-1细胞;联合给药;增殖;凋亡;上皮间质转化
KEYWORDS: Ginsenoside CK; 5-fluorouracil; Human pancreatic cancer PANC-1 cells; Combined administration; Proliferation; Apoptosis; Epithelial mesenchymal transition
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