徐长卿-二妙散-三藤方对蛋白聚糖诱导关节炎模型小鼠血清中TNF-α和DKK-1含量的影响
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篇名: 徐长卿-二妙散-三藤方对蛋白聚糖诱导关节炎模型小鼠血清中TNF-α和DKK-1含量的影响
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摘要: 目的:研究徐长卿-二妙散-三藤方对关节炎模型小鼠血清中肿瘤坏死因子α(TNF-α)和骨化相关因子DKK-1含量的影响,探讨该方治疗关节炎的作用机制。方法:将60只BALB/c小鼠随机分为正常组、模型组、柳氮磺吡啶组(阳性对照,9 mg/kg)和徐长卿-二妙散-三藤方低、中、高剂量组(以生药量计分别为11.25、22.5、45 g/kg),每组10只。除正常组外的其余50只小鼠均采用完全弗氏佐剂+蛋白聚糖ip法建立关节炎模型。成模后,各给药组小鼠ig相应药物,正常组和模型组小鼠ig等体积生理盐水,每天1次,连续20 d。给药结束后,采用酶联免疫吸附法检测各组小鼠血清中TNF-α和DKK-1含量,透射电镜下观察小鼠骶髂关节滑膜细胞超微病理结构变化。结果:与正常组比较,模型组小鼠血清中TNF-α含量明显升高、DKK-1含量明显降低(P<0.05);镜下可见小鼠骶髂关节滑膜细胞发生增生肥大、胞质内细胞器变形、线粒体肿胀等病理损伤。与模型组比较,各给药组小鼠血清中TNF-α含量均明显降低(P<0.05或P<0.01),除柳氮磺吡啶组外的其余各给药组小鼠血清中DKK-1含量均明显升高(P<0.05);镜下可见各给药组小鼠骶髂关节滑膜细胞的病理损伤均有不同程度减轻,且徐长卿-二妙散-三藤方组小鼠的病理损伤好转程度较柳氮磺吡啶组更显著。结论:徐长卿-二妙散-三藤方可能通过降低血清中TNF-α含量和升高DKK-1含量,从而抑制关节炎模型小鼠的骶髂关节炎症和病理性骨化。
ABSTRACT: OBJECTIVE: To study the effect of Xuchangqing-ermiaosan-santeng formula on the contents of tumor necrosis factor α (TNF-α) and ossification-related factor DKK-1 in serum of model mice with arthritis, and reveal its mechanism in the treatment of arthritis. METHODS: 60 BALB/c mice were randomly divided into normal group, model group, sulfasalazine group (positive control, 9 mg/kg) and Xuchangqing-ermiaosan-santeng formula low-dose, medium-dose, high-dose groups (calculated by crude drug as 11.25, 22.5, 45 g/kg), 10 in each group. Except for normal group, other 50 mice were intraperitoneallly injected complete Freund’s adjuvant + proteoglycans to induce model with arthritis. After modeling, mice in each administration group were intragastrically administrated relevant medicines, mice in normal group and model group were intragastrically administrated equal volume of normal saline, once a day, for 20 d. After administration, enzyme-linked immunosorbent assay was used to detect the contents of TNF-α and DKK-1 in serum of mice in each group, and ultrastructural changes of sacroiliac joint synovial cells were observed by transmission electron microscopy. RESULTS: Compared with normal group, TNF-α content in serum in model group was obviously increased, DKK-1 content was obviously decreased (P<0.05); sacroiliac joint synovial cells showed hyperplasia, organellar deformation, mitochondrial swelling and other pathologic damage. Compared with model group, TNF-α contents in serum in each administration group were obviously decreased (P<0.05 or P<0.01); except for sulfasalazine group, the DKK-1 content of mice in other administration groups were obviously increased (P<0.05). Pathologic damages of sacroiliac joint synovial cells in each administration group were reduced to varying degrees, and improvement degree in Xuchangqing-ermiaosan-santeng formula groups was higher than sulfasalazine group. CONCLUSIONS: Xuchangqing-ermiaosan-santeng formula may inhibit the sacroiliac arthritis and pathological ossification of model mice with arthritis by decreasing TNF-α content and increasing DKK-1 content in serum.
期刊: 2017年第28卷第31期
作者: 吴琪,周晓红,杨德才,何敢想,任婕,胡燕芬
AUTHORS: WU Qi,ZHOU Xiaohong,YANG Decai,HE Ganxiang,REN Jie,HU Yanfen
关键字: 徐长卿-二妙散-三藤方;关节炎;肿瘤坏死因子α;骨化相关因子DKK-1;BALB/c小鼠
KEYWORDS: Xuchangqing-ermiaosan-santeng formula; Arthritis; Tumor necrosis factor α; Ossification-related factor DKK- 1; BALB/c mice
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