苗药黑骨藤中咖啡酰基奎宁酸类部位对人类风湿性关节炎成纤维样滑膜细胞MH7A增殖及炎症因子分泌的影响
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篇名: 苗药黑骨藤中咖啡酰基奎宁酸类部位对人类风湿性关节炎成纤维样滑膜细胞MH7A增殖及炎症因子分泌的影响
TITLE:
摘要: 目的:研究苗药黑骨藤中咖啡酰基奎宁酸类部位(CADF)对肿瘤坏死因子α(TNF-α)诱导的人类风湿性关节炎(RA)成纤维样滑膜细胞MH7A增殖及炎症因子分泌的影响,探讨CADF抗RA的作用机制。方法:将MH7A细胞分为空白组、TNF-α模型组、甲氨蝶呤组(阳性对照,20 mg/L)和CADF不同质量浓度组(50、100、200、400 mg/L),除空白组外,其余各组均以50 μg/L TNF-α刺激活化MH7A细胞。以TNF-α与相应药物的混合液共同作用24 h后,检测细胞的增殖情况和培养液中一氧化氮(NO)、前列腺素E2(PGE2)、白细胞介素1β(IL-1β)、白细胞介素6(IL-6)的含量。结果:与空白组比较,TNF-α模型组细胞增殖活性显著增强(P<0.01),培养液中NO、PGE2、IL-1β、IL-6含量显著增加(P<0.01);与TNF-α模型组比较,各给药组细胞的增殖均受到显著抑制(P<0.05或P<0.01),培养液中NO、PGE2、IL-1β、IL-6含量均显著减少(P<0.01),且与CADF具有一定的量效关系。结论:CADF可通过抑制TNF-α诱导的MH7A细胞增殖,减少炎症因子NO、PGE2、IL-1β、IL-6的分泌,从而发挥其抗RA的作用。
ABSTRACT: OBJECTIVE: To study the effects of caffeoylquinic acid derivative fractions (CADF) in Miao medicine Periploca forrestii on proliferation and secretion of inflammatory cytokines in tumor necrosis factor α (TNF-α)-induced human rheumatoid arthritis (RA) fibroblast-like synoviocytes MH7A, and explore its mechanism on anti-RA. METHODS: MH7A cells were divided into blank group, TNF-α model group, methotrexate group (positive control, 20 mg/L) and CADF different mass concentrations groups (50, 100, 200, 400 mg/L). Except for blank group, other groups received 50 μg/L of TNF-α to stimulate MH7A cells. After treated by suspension with TNF-α and related medicines for 24 h, the cell proliferation and contents of nitric oxide (NO), prostaglandin E2 (PGE2), interleukin 1β (IL-1β), interleukin 6 (IL-6) in culture medium were detected. RESULTS: Compared with blank group, cell proliferation activity in TNF-α model group was significantly enhanced (P<0.01), contents of NO, PGE2, IL-1β, IL-6 in culture medium were significantly increased (P<0.01). Compared with TNF-α model group, cell proliferation in each administration group were significantly inhibited (P<0.05 or P<0.01), contents of NO, PGE2, IL-1β, IL-6 in culture medium were significantly decreased (P<0.01), showing certain dose-effect relationship with CADF. CONCLUSIONS: CADF can play the role in anti-RA by inhibiting the TNF-α-induced proliferation of MH7A cells and reducing the secretion of inflammatory cytokines NO, PGE2, IL-1β, IL-6.
期刊: 2017年第28卷第28期
作者: 王霞,杨健,宋菲,董莉,刘亭,郑林,马雪,李勇军
AUTHORS: WANG Xia,YANG Jian,SONG Fei,DONG Li,LIU Ting,ZHENG Lin,MA Xue,LI Yongjun
关键字: 苗药;黑骨藤;咖啡酰基奎宁酸类部位;人类风湿性关节炎成纤维样滑膜细胞MH7A;炎症因子
KEYWORDS: Miao medicine; Periploca forrestii; Caffeoylquinic acid derivative fractions; Human rheumatoid arthritis fibroblast-like synoviocytes MH7A; Inflammatory cytokines
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