葛根素立方液晶的制备及在微针作用下的体外透皮特性研究
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篇名: 葛根素立方液晶的制备及在微针作用下的体外透皮特性研究
TITLE:
摘要: 目的:制备葛根素立方液晶,研究其在微针作用下的体外透皮特性。方法:以单油酸甘油酯、乙醇、葛根素、纯化水为材料,采用注入法制备葛根素立方液晶;用偏光显微镜和小角X射线衍射进行表征。采用改进的Franz扩散池比较葛根素10%乙醇水溶液和葛根素立方液晶的体外透皮特性;考察葛根素立方液晶在微针作用下的体外透皮特性。结果:所制葛根素立方液晶在偏光显微镜下无明显晶体分布,呈暗绿色,显各向同性;其散射峰位之比为[√6]  ∶ [√8] ,证明其内部结构为螺旋型晶格。葛根素10%乙醇水溶液和葛根素立方液晶的透皮速率分别为15.306、29.101 μg/(cm2·h),20 h内的累积透皮量分别为190、545 μg/cm2;200 μm微针预处理皮肤后,葛根素立方液晶的透皮速率为78.15 μg/(cm2·h),20 h内的累积透皮量为1 450 μg/cm2。结论:成功制得葛根素立方液晶,其具有较葛根素乙醇水溶液更强的透皮能力,且联合微针给药具有更好的透皮性能。
ABSTRACT: OBJECTIVE: To prepare the puerarin cubic phase, and study its in vitro transdermal characteristics under microneedle. METHODS: Using glycerol monooleate, ethanol, puerarin and purified water as materials, injection method was used to prepare the puerarin cubic phase. Polarizing microscopy and small angle X-ray diffraction were used for characterizing. The in vitro transdermal characteristics of Puerarin 10% ethanol solution and puerarin cubic phase were comparatively studied by modified Franz diffusion cell, and the in vitro transdermal characteristics of puerarin cubic phase under microneedle were studied. RESULTS: Prepared puerarin cubic phase had no obvious phase distribution under polarizing microscope with dark green color, showing isotropic trait. The ratio of scattering peak position was [√6]  ∶ [√8] , indicating that its internal structure was spiral lattice. The transdermal rates of Puerarin 10% ethanol solution and puerarin cubic phase were 15.306, 29.101 μg/(cm2·h), and the cumulative transdermal amounts within 20 h were 190, 545 μg/cm2, respectively. After the pretreatment on skin by 200 μm microneedle, the transdermal rates of puerarin cubic phase was 78.15 μg/(cm2·h), and the cumulative transdermal amounts within 20 h was 1 450 μg/cm2. CONCLUSIONS: Puerarin cubic phase is successfully prepared, which shows stronger transdermal ability than puerarin ethanol aqueous solution and better transdermal ability when combined with microneedle administration.
期刊: 2017年第28卷第22期
作者: 莫建民
AUTHORS: MO Jianmin
关键字: 葛根素;立方液晶;制备;微针;体外透皮
KEYWORDS: Puerarin; Cubic phase; Preparation; Microneedle; in vitro transdermal
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