HPCE法测定金银花药材中木犀草苷、槲皮素和金丝桃苷的含量
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篇名: HPCE法测定金银花药材中木犀草苷、槲皮素和金丝桃苷的含量
TITLE:
摘要: 目的: 建立同时测定金银花药材中木犀草苷、槲皮素和金丝桃苷含量的方法。方法:采用高效毛细管电泳法。毛细管柱为石英毛细管柱,检测波长为360 nm,分离电压为20 kV,进样方式为电动进样,进样电压为15 kV,进样时间为5 s,操作温度为25 ℃,缓冲溶液为60 mmol/L 四硼酸钠-50 mmol/L碳酸钠-50 mmol/L 丙羟基-β-环糊精(pH 9.2)。结果:木犀草苷、槲皮素和金丝桃苷检测质量浓度线性范围分别为0.06~0.56 mg/mL(r=0.988 1)、0.08~0.56 mg/mL(r=0.989 2)、0.06~0.49 mg/mL(r=0.979 6);精密度、稳定性、重复性的RSD<2.0%;加样回收率分别为96.12%~99.77%(RSD=1.29%,n=6)、95.90%~98.35%(RSD=0.89%,n=6)、94.07%~97.45%(RSD=1.33%,n=6)。结论:该方法操作简便,精密度、稳定性、重复性好,可用于金银花药材中木犀草苷、槲皮素和金丝桃苷含量的同时测定。
ABSTRACT: OBJECTIVE: To establish a method for contents determination of luteoloside, quercetin and hyperoside in Lonicera japonica. METHODS: HPCE was performed silica capillary column with detection wavelength of 360 nm and separation voltage of 20 kV, electrokinetic sampling, sampling voltage of 15 kV, sampling time of 5 s, operation temperature of 25 ℃.The buffer was consisted of 60 mmol/L sodium tetraborate-50 mmol/L natrium carbonicum-50 mmol/L hydroxypropyl-β-cyclodextrin (pH 9.2). RESULTS: The linear ranges of luteoloside, quercetin and hyperoside were 0.06-0.56mg/mL (r=0.988 1), 0.08-0.56 mg/mL (r=0.989 2), 0.06-0.49 mg/mL (r=0.979 6), respectively. RSDs of precision, stability and reproducibility tests were all lower than 2.0%. The recoveries were 96.12%-99.77%(RSD=1.29%,n=6), 95.90%-98.35%(RSD=0.89%,n=6), 94.07%-97.45%(RSD=1.33%,n=6), respectively. CONCLUSIONS: The method is simple, accurate, stable and reproducible, and can be used for simultaneous determination of luteoloside, quercetin and hyperoside in L. japonica.
期刊: 2017年第28卷第18期
作者: 曾雪,杨元娟,陈竹,唐倩,石磊
AUTHORS: ZENG Xue,YANG Yuanjuan,CHEN Zhu,TANG Qian,SHI Lei
关键字: 高效毛细管电泳法;木犀草苷;槲皮素;金丝桃苷;金银花
KEYWORDS: HPCE; Luteoloside; Quercetin; Hyperoside; Lonicera japonica
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