金银花炒炭前后6种成分的含量变化研究
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篇名: 金银花炒炭前后6种成分的含量变化研究
TITLE:
摘要: 目的:建立同时测定金银花中6种成分含量的方法,并研究金银花炒炭前后上述成分含量的变化。方法:采用超高效液相色谱法。色谱柱为Agilent Eclipse Plus C18 RRHD,流动相为0.1%磷酸溶液-乙腈(梯度洗脱),流速为0.2 mL/min,检测波长为350 nm,柱温为25 ℃,进样量为1 μL。结果:绿原酸、芦丁、木犀草苷、异绿原酸A、异绿原酸B、异绿原酸C 检测进样量线性范围分别为21.2~424 μg(r=0.999 3)、1.17~23 μg(r=0.999 5)、2.18~43 μg(r=0.999 8)、5.10~102 μg(r=0.999 3)、2.60~52 μg(r=0.999 1)、4.95~99 μg(r=0.999 8);精密性、稳定性、重复性试验的RSD<2.0%;加样回收率分别为97.11%~99.76%(RSD=1.20%,n=6)、95.20%~99.90%(RSD=2.20%,n=6)、95.71%~100.30%(RSD=2.20%,n=6)、95.00%~96.98%(RSD=0.88%,n=6)、96.47%~103.00%(RSD=2.40%,n=6)、95.78%~103.80%(RSD=3.20%,n=6)。与炮制前比较,炮制后的金银花中芦丁、异绿原酸B、异绿原酸C的含量增加,绿原酸、异绿原酸A的含量明显下降,而木犀草苷的含量无明显变化。结论:该方法操作简便,精密度、稳定性、重复性好,可用于金银花中6种成分含量的同时测定;金银花炒炭前后化学成分有一定变化。
ABSTRACT: OBJECTIVE: To establish the method for simultaneous determination of 6 components in Lonicera japonica, and to study the content changes of them before and after before and after carbonized. METHODS: UPLC method was adopted. The determination was performed on Agilent Eclipse Plus C18 RRHD column with mobile phase consisting of 0.1% phosphoric acid solution-acetonitrile (gradient elution) at the flow rate of 0.2 mL /min. The detection wavelength was set at 350 nm, and column temperature was 25 ℃. The sample size was 1 μL. RESULTS: The linear ranges of chlorogenic acid, rutin, galuteolin, isochlorogenic acid A, isochlorogenic acid B and isochlorogenic acid C were 21.2-424 μg(r=0.999 3), 1.17-23 μg(r=0.999 5), 2.18-43 μg(r=0.999 8), 5.10-102 μg(r=0.999 3), 2.60-52 μg(r=0.999 1), 4.95-99 μg(r=0.999 8), respectively. RSDs of precision, stability and repeatability tests were all lower than 2.0%. Recoveries were 97.11%-99.76%(RSD=1.20%,n=6), 95.20%-99.90%(RSD=2.20%,n=6), 95.71%-100.30%(RSD=2.20%,n=6), 95.00%-96.98%(RSD=0.88%,n=6), 96.47%-103.00%(RSD=2.40%,n=6), 95.78%-103.80%(RSD=3.20%,n=6). Compared with before processing, the contents of rutin, isochlorogenic acid B and isochlorogenic acid C in L. japonica were increased along with processing, the contents of chlorogenic acid and isochlorogenic acid A were decreased significantly, while the content of galuteolin had no significant change. CONCLUSIONS: The method is simple, precise, stable and repeatable, and can be used for simultaneous determination of 6 components in L. japonica. Those chemical components have certain changes before and after carbonized.
期刊: 2017年第28卷第15期
作者: 吴明侠,李会,崔永霞,侯珊珊,丁亚慧
AUTHORS: WU Mingxia,LI Hui,CUI Yongxia,HOU Shanshan,DING Yahui
关键字: 金银花;炒炭;超高效液相色谱法;含量;变化
KEYWORDS: Lonicera japonica; Carbonize; UPLC; Content; Change
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