绿萼梅总黄酮对慢性应激抑郁模型大鼠抑郁行为的影响及机制研究
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篇名: 绿萼梅总黄酮对慢性应激抑郁模型大鼠抑郁行为的影响及机制研究
TITLE:
摘要: 目的:考察绿萼梅总黄酮对慢性应激抑郁模型大鼠抑郁行为的影响及机制。方法:将60只大鼠随机分为生理盐水组、模型组、氟西汀组(阳性对照,20 mg/kg)和绿萼梅总黄酮低、中、高剂量组(80、160、240 mg/kg),每组10只。除生理盐水组外,其余各组大鼠均采用慢性不可预见性温和应激(CUMS)+孤养法复制抑郁模型,并于造模同时ig相应药物,每天1次,连续28 d。观察大鼠体质量、摄食量的变化及糖水偏爱程度;通过强迫游泳实验、悬尾实验测定大鼠不动时间以及旷场实验测定大鼠中央格停留时间、水平穿越格数、站立次数、修饰次数的变化;并测定末次给药24 h后大鼠血清中肿瘤坏死因子α(TNF-α)、皮质醇、白细胞介素6(IL-6)、5-羟色胺(5-HT)水平。结果:与生理盐水组比较,模型组大鼠的体质量增加值、摄食量、糖水偏爱百分比均降低;强迫游泳和悬尾实验的不动时间延长;旷场实验的中央格停留时间延长、水平穿越格数、站立次数、修饰次数均减少;血清中TNF-α、皮质醇水平升高,IL-6、5-HT水平降低,差异均有统计学意义(P<0.05或P<0.01)。与模型组比较,除绿萼梅总黄酮低剂量组大鼠糖水偏爱百分比、强迫游泳不动时间、旷场实验修饰次数以及绿萼梅总黄酮低、中剂量组大鼠血清中IL-6、5-HT水平改善不明显外,其余各给药组大鼠上述指标均明显改善(P<0.05或P<0.01)。结论:绿萼梅总黄酮能明显改善CUMS诱导的大鼠抑郁行为,其机制可能与抑制炎症反应、调节下丘脑-垂体-肾上腺轴功能有关。
ABSTRACT: OBJECTIVE: To investigate the effects and mechanism of total flavonoids from Armeniaca mume on depression in chronic stress depression model rats. METHODS: 60 rats were randomly divided into normal saline group, model group, fluoxetine group (positive control, 20 mg/kg) and total flavonoids from A. mume low-dose, medium-dose, high-dose groups (80, 160, 240 mg/kg), 10 in each group. Except for normal saline group, the other groups adopted chronic unpredictable mild stress (CUMS)+solitary feeding condition to induce depression model. These groups were intragastrically administered, once a day, for 28 d. Changes of body mass and food intake, degree of preference for sugar were observed; forced swimming test and tail suspension test were used to determine the time that rats did not move; open field test was used to determine the changes of residence time in central square, horizontal crossing lattice, standing times, modification times; tumor necrosis factor α (TNF-α), cortisol, interleukin 6 (IL-6), serotonin (5-HT) levels in serum after 24 h of last administration were determined. RESULTS: Compared with normal saline group, growth of body mass, food intake and sugar preference percentage in model group were decreased; the time that rats did not move was prolonged in forced swimming test and tail suspension test; residence time in central square was prolonged, while horizontal crossing lattice, standing times and modification times were decreased in open field test; serum levels of TNF-α, cortisol were increased, while IL-6, 5-HT were decreased, with statistical significances (P<0.05 or P<0.01). Compared with model group, except that there were no obvious improvement in sugar preference percentage, the time that rats did not move in forced swimming test, modification times in open field test in total flavonoids from A. mume low-dose group and IL-6, 5-HT levels in serum in total flavonoids from A. mume low-dose, medium-dose groups, the above-mentioned indexes were obviously improved in other groups (P<0.05 or P<0.01). CONCLUSIONS: Total flavonoids from A. mume can obviously improve the CUMS-induced depression in rats, and the mechanism may be related to inhibiting inflammation response, adjusting the hypothalamus-pituitary-adrenal axis functions.
期刊: 2017年第28卷第13期
作者: 陈明珠,陈静,黄幼霞,程晶,廖婉婷
AUTHORS: CHEN Mingzhu,CHEN Jing,HUANG Youxia,CHENG Jing,LIAO Wanting
关键字: 绿萼梅总黄酮;慢性不可预见性温和应激;抑郁模型;炎症因子;行为学;大鼠
KEYWORDS: Total flavonoids from Armeniaca mume; Chronic unpredictable mild stress; Depression model; Inflammation factor; Behavioral science; Rats
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