枳实及其主要活性成分促进脾虚模型大鼠胃肠运动的机制研究
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篇名: 枳实及其主要活性成分促进脾虚模型大鼠胃肠运动的机制研究
TITLE:
摘要: 目的:研究枳实及其主要活性成分促进脾虚模型大鼠胃肠运动的机制。方法:将170只大鼠随机分为空白组(10只)和造模组(160只),造模组大鼠以苦寒泻下+饥饱失常法复制脾虚模型。造模后,将大鼠随机分为模型组,柚皮苷低、中、高剂量组(3.267、6.535、13.070 mg/mL),新橙皮苷低、中、高剂量组(3.865、7.730、15.460 mg/mL),辛弗林低、中、高剂量组(0.252、0.504、1.008 mg/mL),3个单体成分(即柚-新-辛)低、中、高剂量配伍组和枳实水煎液低、中、高剂量组(0.104、0.208、0.416 g/mL,以生药计),每天ig给药1次,10 mL/kg,连续7 d。末次给药后,检测大鼠血清胃泌素(GAS)和血浆乙酰胆碱(ACh)、胃动素(MTL)、P物质(SP)、血管活性肠肽(VIP)水平。结果:与空白组比较,模型组大鼠血清GAS水平和血浆ACh、MTL、SP水平降低(P<0.01),血浆VIP水平升高(P<0.05)。与模型组比较,除柚皮苷高剂量组和辛弗林各剂量组大鼠血清GAS水平外,其余各给药组均明显升高(P<0.05或P<0.01);新橙皮苷高剂量组、辛弗林高剂量组和枳实水煎液低剂量组大鼠血浆ACh水平显著升高(P<0.01);新橙皮苷中、高剂量组,辛弗林高剂量组,低、中剂量配伍组和枳实水煎液中、高剂量组大鼠血浆MTL水平显著升高(P<0.05);柚皮苷低、中剂量组和新橙皮苷各剂量组大鼠血浆SP水平显著升高(P<0.05或P<0.01);柚皮苷低剂量组,辛弗林高剂量组和枳实水煎液低、中剂量组大鼠血浆VIP水平显著降低(P<0.05)。结论:枳实可能通过促进GAS、ACh、MTL的分泌和抑制VIP的分泌来促进脾虚模型大鼠的胃肠运动;其与3个单体成分对胃肠激素的调节作用有差异。
ABSTRACT: OBJECTIVE: To study the mechanism of Aurantii fructus immaturus (AFI) and its main active ingredients in promoting gastrointestinal motility of model rats with spleen deficiency. METHODS: 170 rats were randomly divided into blank group (10 rats) and modeling group (160 rats), rats in modeling group was induced models with spleen deficiency by bitter cold diarrhea+irregular diet. After modeling, rats were randomly divided into model group, naringin (NA) low-dose, medium-lose, high-dose groups (3.267, 6.535, 13.070 mg/mL), neohesperidin (NE) low-dose, medium-lose, high-dose groups (3.865, 7.730, 15.460 mg/mL), synephrine (SY) low-dose, medium-lose, high-dose groups (0.252, 0.504, 1.008 mg/mL), compatibility groups with 3 monomer ingredients (NA-NE-SY) low-dose, medium-lose, high-dose and AFI water decoction low-dose, medium-lose, high-dose groups (0.104, 0.208, 0.416 g/mL, calculated by crude drug), ig, once a day, 10 mL/kg, for 7 d. After the last administration, gastrin (GAS) in serum, and acetylcholine (ACh), motilin (MTL), substance P (SP), vasoactive intestinal peptide (VIP) levels in plasma were detected. RESULTS: Compared with blank group, GAS level in serum and ACh, MTL, SP levels in plasma in model group were reduced (P<0.01), VIP level in plasma was increased (P<0.05). Compared with model group, except for the GAS level in serum showed no obvious change in NA high-dose group and SY doses groups, other medicine groups were obviously increased (P<0.05 or P<0.01); the ACh levels in serum were obviouly increased in NE high-dose group, SY high-dose group and AFI water decoction low-dose group (P<0.01). MTL levels in plasma were obviously increased in NE medium-dose, high-dose groups, SY high-dose group, compatibility low-dose, medium-dose groups and AFI water decoction medium-dose, high-lose groups (P<0.05); SP levels in plasma were obviously increased in NA low-dose, medium-dose groups and NE doses groups (P<0.05 or P<0.01); VIP levels were reduced in NA low-dose group, SY high-dose group and AFI water decoction low-dose, medium-lose groups (P<0.05). CONCLUSIONS: AFI may promote the gastrointestinal motility of model rats with spleen deficiency by promoting the secretion of GAS, ACh, MTL, and inhibiting the secretion of VIP; there are differences between AFI and the 3 monomer ingredients in regulation of gastrointestinal hormones.
期刊: 2017年第28卷第13期
作者: 胡源祥,陈海芳,宋玉鹏,谭舒舒,罗小泉,杨武亮
AUTHORS: HU Yuanxiang,CHEN Haifang,SONG Yupeng,TAN Shushu,LUO Xiaoquan,YANG Wuliang
关键字: 枳实;柚皮苷;新橙皮苷;辛弗林;胃肠激素;脾虚模型;大鼠
KEYWORDS: Aurantii fructus immaturus; Naringin; Neohesperidin; Synephrine; Gastrointestinal hormones; Spleen deficiency model; Rats
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