紫叶李果实总黄酮对大鼠酒精性肝损伤的保护作用机制研究
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篇名: 紫叶李果实总黄酮对大鼠酒精性肝损伤的保护作用机制研究
TITLE:
摘要: 目的:研究紫叶李果实总黄酮(PCE)对酒精性肝损伤(ALD)大鼠的保护作用机制,为将其开发为治疗ALD的新药提供实验依据。方法:将40只大鼠随机分为正常组(蒸馏水)、模型组(蒸馏水)、水飞蓟宾组[阳性对照,30 mg/(kg·d)]和PCE高、低剂量组[80、40 mg/(kg·d)],每组8只。每天上午ig给药(10 mL/kg)1次,持续6周;给药同时,除正常组外其余各组大鼠每天下午ig 50%乙醇(10 mL/kg)1次诱导ALD模型。给药结束后,测定大鼠心、肝、脾等脏器指数和皮下脂肪、棕色脂肪、腹腔脂肪指数;测定谷氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、总胆红素(TBIL)、直接胆红素(DBIL)、间接胆红素(IBIL)、γ-谷氨酰转肽酶(GGT)以及总胆固醇(TC)、三酰甘油(TG)、高密度脂蛋白(HDL)、低密度脂蛋白(LDL)等血清生化指标水平;测定肝组织中超氧化物歧化酶(SOD)、丙二醛(MDA)和谷胱甘肽过氧化酶(GSH-Px)、TC、TG以及粪便中TC、TG等生化指标水平;观察大鼠肝、肾组织病理变化。结果:与正常组比较,模型组大鼠肝、脾、肾指数和皮下脂肪、腹腔脂肪指数升高(P<0.05或P<0.01),脑指数和棕色脂肪指数降低(P<0.05);血清中HDL水平和HDL/TC比值降低,其余血清指标均升高(P<0.05或P<0.01);肝组织中SOD、GSH-Px水平降低,其余肝组织生化指标均升高(P<0.01);粪便中TC、TG水平升高(P<0.01);肝、肾均发生明显病变。与模型组比较,水飞蓟宾组和PCE高剂量组大鼠上述指标均显著改善(P<0.05或P<0.01);PCE低剂量组大鼠血清中ALT、AST和肝组织中MDA水平显著降低(P<0.01),肝组织中SOD和GSH-Px水平显著升高(P<0.05);各给药组肝、肾病变程度及肝中脂质聚积减轻。结论:PCE可能通过抗氧化、促进肝细胞再生、调节脂质代谢等方面发挥其改善ALD的作用。
ABSTRACT: OBJECTIVE: To study the protective effects of total flavone from Prunus cerasifera fruits (PCE) on alcoholic liver disease (ALD) in rats, and provide experimental basis for developing new medicines for anti-ALD. METHODS: 40 rats were randomly divided into normal group (distilled water), model group (distilled water), silibinin group [positive control, 30 mg/(kg·d)] and PCE high-dose, low-dose groups [80, 40 mg/(kg·d)], 8 in each group. All rats were intragastrically administrated (10 mL/kg) every morning, once, for 6 weeks; meanwhile, except for normal group, rats in other groups received 50% alcohol (10 mL/kg) once intragastrically every afternoon to induce ALD model. After administration, heart, liver, spleen and other organ indexes, subcutaneous fat, brown fat, abdominal fat indexes were determined, as well as serum biochemical indexes [glutamate transaminase (ALT), aspartate aminotransferase (AST), total bilirubin (TBIL), direct bilirubin (DBIL), indirect bilirubin (IBIL), gamma-glutamyl transpeptidase (GGT), and total cholesterol (TC), triglyceride (TG), high-density lipoprotein (HDL), low-density lipoprotein (LDL)] level and liver biochemical indexes [superoxide dismutase (SOD), malondialdehyde (MDA), glutathione peroxidase (GSH-Px), TC, TG] and TC, TG levels in feces; pathological changes of liver and kidney tissues were observed. RESULTS: Compared with normal group, heart, liver, spleen indexes, subcutaneous fat, abdominal fat indexes in model group were increased (P<0.05 or P<0.01), brain index and brown fat index were decreased (P<0.05); HDL level and HDL/TC ratio in serum were decreased, other serum indexes were increased (P<0.05 or P<0.01); SOD, GSH-Px levels in liver tissue were decreased, other above-mentioned liver biochemical indexes were increased (P<0.01); TC, TG levels in feces were increased (P<0.01); liver and kidney showed obvious lesions. Compared with model group, the above-mentioned indexes in silibinin group and PCE high-dose group were significantly improved (P<0.05 or P<0.01); ALT, AST in serum and MDA level in liver tissue in PCE low-dose group were significantly decreased (P<0.01), and SOD, GSH-Px levels in liver tissue were significantly increased (P<0.05); lesion degree of liver and kidney and lipid accumulation in liver were reduced in administration groups. CONCLUSIONS: PCE may play a role in anti-ALD by anti-oxidation, promoting liver cell regeneration and regulating lipid metabolism.
期刊: 2017年第28卷第10期
作者: 冯海容,郑轶,常海茹,赵永会
AUTHORS: FENG Hairong,ZHENG Yi,CHANG Hairu,ZHAO Yonghui
关键字: 紫叶李果实总黄酮;酒精性肝损伤;保护作用;机制;大鼠
KEYWORDS: Total flavone from Prunus cerasifera fruits; Alcoholic liver disease; Protective effect; Mechanism; Rats
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