正交试验优化白术配方颗粒的索氏提取工艺
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篇名: 正交试验优化白术配方颗粒的索氏提取工艺
TITLE:
摘要: 目的:优化白术药材的索氏提取工艺,为其配方颗粒的研制提供依据。方法:以白术内酯Ⅰ、Ⅱ、Ⅲ的含量为指标,在单因素试验基础上,以提取时间、料液比、提取次数为因素,采用L9(34)正交试验,优化及验证白术配方颗粒的索氏提取工艺并与其他3种工艺(常温浸提法、超声提取法、回流提取法)进行比较。结果:优化的提取工艺为用6倍药材量乙醇提取3次,共提取8 h;验证试验结果显示,白术内酯3次提取量分别为0.769 、0.752、0.781 mg/g(RSD=1.99%,n=3),均高于其他3种方法的提取量(0.683、0.489、0.693 mg/g)。结论:优化后的提取方法对白术内酯Ⅰ、Ⅱ、Ⅲ的提取效率较高,可用于配方颗粒制备时白术中内酯成分的提取。
ABSTRACT: OBJECTIVE: To optimize the soxhlet extraction technology of Atractylodis macrocephalae, and to provide evidence for research and preparation of its formula granules. METHODS: Using the contents of atractylenolide Ⅰ, Ⅱ, Ⅲ as index, based on single factor test, the Soxhlet extraction technology of A. macrocephalae formula granules was optimized and verified by L9(34) orthogonal test with extraction time, solid-liquid ratio, extraction times as factors, and then compared with other technologies (normal temperature extraction method, ultrasonic extraction method, reflux extraction method). RESULTS: The optimal extraction technology was as follows as 6-fold ethanol, extracting for 3 times, lasting for 8 h. Results of validation test showed that the extraction amounts of atractylenolide were 0.769, 0.752, 0.781 mg/g (RSD=1.99%, n=3) for 3 times, which were higher than the extraction amounts of other 3 methods (0.683, 0.489, 0.693 mg/g). CONCLUSIONS: The optimized extraction technology possesses high extraction rates of atractylenolide Ⅰ, Ⅱ, Ⅲ, and can be used for the extraction of internal ether from A. macrocephalae formula granules.
期刊: 2017年第28卷第7期
作者: 李功华,李洪玉,费莹,寿旦
AUTHORS: LI Gonghua,LI Hongyu,FEI Ying,SHOU Dan
关键字: 白术;白术内酯;索氏提取;工艺优化;正交试验
KEYWORDS: Atractylodis macrocephalae; Atractylenolide; Soxhlet extraction; Technology optimization; Orthogonal test
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