三黄益肾方的纯化工艺研究
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篇名: 三黄益肾方的纯化工艺研究
TITLE:
摘要: 目的:研究三黄益肾方的纯化工艺。方法:以纯化后澄清液中总多糖、黄芪甲苷、毛蕊异黄酮葡萄糖苷保留率和除杂率为指标,分别考察水提醇沉法(50%、60%、70%乙醇)和澄清剂法(101果汁澄清剂、ZTC天然澄清剂、壳聚糖澄清剂)的纯化效果,以筛选三黄益肾方纯化方法;设计正交试验对最优纯化方法的工艺参数(药液质量浓度、澄清剂用量和药液pH)进行优化,并进行验证试验。结果:纯化方法中以壳聚糖为澄清剂时纯化效果较优,综合评分为98.62;优化后工艺参数为药液质量浓度1 g/mL、1%壳聚糖溶液用量2 mL/g、pH 5.1;验证试验中总多糖、黄芪甲苷、毛蕊异黄酮葡萄糖苷保留率和除杂率平均值分别为79.56%、78.11%、79.46%、32.18%(RSD分别为1.24%、0.97%、1.03%、1.16%,n=3)。结论:采用壳聚糖为澄清剂对三黄益肾方纯化效果较好,且操作简单,优化后工艺稳定、可行。
ABSTRACT: OBJECTIVE: To study the purification technology of Sanhuang yishen formula. METHODS: Using retention rate and impurity rate of purified total polysaccharide, astragaloside and calycosin glucoside as index, the purification effects of water extraction and alcohol precipitation method (50%, 60%, 70% ethanol) and clarifying agent method (101 juice clarifying agent, ZTC natural clarifying agent, chitosan clarifying agent) were respectively detected to screen the purification method; orthogonal test was used to optimize the technology parameters (mass concentration of liquid, amount of clarifying agent and pH of liquid) by the optimized purification method, and the verification test was conducted. RESULTS: The purification was better when using chitosan as clarifying agent with comprehensive score of 98.62; the purified technology parameters were mass concentration of liquid 1 g/mL, 1% chitosan solution amount of 2 mL/g, pH 5.1; the average value of retention rate and impurity rate of purified total polysaccharide, astragaloside and calycosin glucoside in verification test were 79.56% (RSD=1.24%, n=3), 78.11% (RSD=0.97%, n=3), 79.46% (RSD=1.03%, n=3) and 32.18% (RSD=1.16%, n=3), respectively. CONCLUSIONS: Using chitosan as clarifying agent shows good purification effect for Sanhuang yishen formula, which is simple. The optimized technology is stable and feasible.
期刊: 2017年第28卷第7期
作者: 吴小斌,王洛临,李洁环,钟如帆,袁柳萍,郭鸣
AUTHORS: WU Xiaobin,WANG Luolin,LI Jiehuan,ZHONG Rufan,YUAN Liuping,GUO Ming
关键字: 三黄益肾方;纯化工艺;正交试验;黄芪甲苷;总多糖;毛蕊异黄酮葡萄糖苷;壳聚糖;澄清剂
KEYWORDS: Sanhuang yishen formula; Purification technology; Orthogonal test; Astragaloside; Total polysaccharide; Calycosin glucoside; Chitosan; Clarifying agent
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