重组人p53腺病毒注射液对肾母细胞瘤细胞增殖、凋亡及自噬的影响
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篇名: 重组人p53腺病毒注射液对肾母细胞瘤细胞增殖、凋亡及自噬的影响
TITLE:
摘要: 目的:研究重组人p53腺病毒注射液对肾母细胞瘤细胞增殖、凋亡及自噬的影响。方法:将肾母细胞瘤细胞分别与高、中、低浓度(1×109、1×108、1×107 VP/mL)的重组人p53腺病毒注射液共同孵育20 h,以不加注射液的细胞为空白对照。检测细胞增殖、周期、凋亡及相关基因p21、Bax蛋白表达,检测细胞中自噬相关基因LC-3、Atg7、Atg12表达和自噬体数量。结果:高、中、低浓度重组人p53腺病毒注射液对肾母细胞瘤细胞的增殖抑制率分别为(42.86±3.18)%、(33.64±7.25)%、(16.26±9.07)%,细胞凋亡率分别为(53.85±9.36)%、(37.35±9.64)%、(23.64±10.65)%。与空白对照比较,高、中、低浓度药物作用下G0/G1期细胞均增加,其中高、中浓度药物效果较明显(P<0.05或P<0.01);高浓度下细胞中p21、Bax蛋白和LC-3、Atg7、Atg12基因表达增强,自噬体数量增加(P<0.01);中浓度下细胞中Bax蛋白表达增强(P<0.05),其余指标无明显变化(P>0.05)。结论:重组人p53腺病毒注射液可抑制肾母细胞瘤细胞增殖,诱导细胞凋亡和自噬。
ABSTRACT: OBJECTIVE: To study the effects of rAd-p53 injection on the proliferation, apoptosis and autophagy of nephroblastoma cells. METHODS: Nephroblastoma cells were respectively cultured 20 h with high, medium, low concentration (1×109, 1×108, 1×107 VP/mL) of Recombinant human rAd-p53 injection, cells with no injection were the blank control. Cell proliferation, cycle, apoptosis and related gene p21, Bax protein expressions were detected, and autophagy gene LC-3, Atg7, Atg12 expressions and number of autophagosomes were also detected. RESULTS: The proliferation inhibition rates of high, medium, low concentration of Recombinant human rAd-p53 injection to nephroblastoma cells were (42.86±3.18)%, (33.64±7.25)%, (16.26±9.07)%; apoptotic rates were (53.85±9.36)%, (37.35±9.64)%, (23.64±10.65)%, respectively. Compared with blank control, cells at period G0/G1 were increased under high, medium, low concentration Recombinant human rAd-p53 injection, effects were relatively obvious under high, medium concentration (P<0.05 or P<0.01); p21, Bax protein and LC-3, Atg7, Atg12 gene expressions were enhanced under high concentration, the number of autophagosomes was increased (P<0.01); Bax protein expression was enhanced under medium concentration (P<0.05), the other indicators had no obvious changed (P>0.05). CONCLUSIONS: Recombinant human rAd-p53 injection can inhibit the cell proliferation of nephroblastoma, and induce its apoptosis and autophagy.
期刊: 2017年第28卷第7期
作者: 仲智勇,时保军,周辉,王文博
AUTHORS: ZHONG Zhiyong,SHI Baojun,ZHOU Hui,WANG Wenbo
关键字: 肾母细胞瘤;重组人p53腺病毒注射液;细胞周期;增殖;凋亡;自噬
KEYWORDS: Nephroblastoma; rAd-p53 injection; Cell cycle; Proliferation; Apoptosis; Autophagy
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