HPLC法同时测定复方三嗪芦丁片中5种成分的含量
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篇名: HPLC法同时测定复方三嗪芦丁片中5种成分的含量
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摘要: 目的:建立同时测定复方三嗪芦丁片中维生素B6、氢氯噻嗪、芦丁、盐酸异丙嗪、利血平含量的方法。方法:采用高效液相色谱法。色谱柱为ZORBAX SB-C18,流动相为乙腈-0.2%磷酸溶液(梯度洗脱),流速为1.5 ml/min,检测波长为291 nm(0~3 min,维生素B6)、273 nm(3~5 min,氢氯噻嗪)、251 nm(5~11 min,芦丁和盐酸异丙嗪)、218 nm(11 min,利血平),柱温为30 ℃,进样量为20 μl。结果:维生素B6、氢氯噻嗪、芦丁、盐酸异丙嗪和利血平检测质量浓度线性范围分别为84.0~1 344.0 μg/ml(r=0.999 8)、171.0~2 736.0 μg/ml(r=0.999 2)、428.0~6 848.0 μg/ml(r=0.999 9)、173.5~2 776.0 μg/ml(r=0.999 5)、5.03~80.48 μg/ml(r=0.999 1);定量限分别为18.80、14.92、25.70、12.20、4.99 ng,检测限分别为5.54、4.50、8.04、3.66、1.50 ng;精密度、稳定性、重复性试验的RSD<1.0%;加样回收率分别为98.20%~100.60%(RSD=0.89%,n=6)、99.11%~100.28%(RSD=0.39%,n=6)、99.07%~100.12%(RSD=0.38%,n=6)、98.27%~99.70%(RSD=0.50%,n=6)、97.41%~99.40%(RSD=0.88%,n=6)。结论:该方法简便、准确、灵敏度高、重复性好,可用于同时测定复方三嗪芦丁片中维生素B6、氢氯噻嗪、芦丁、盐酸异丙嗪、利血平的含量。
ABSTRACT: OBJECTIVE: To establish a method for the simultaneous determination of vitamin B6, hydrochlorothiazide, rutin, promethazine hydrochloride and reserpine in Compound trizin and tutinum tablet. METHODS: HPLC was performed on the column of Agilent Zorbax SB-C18 with mobile phase of acetonitrile-0.2% phosphoric acid (gradient elution) at a flow rate of 1.5 ml/min, detection wavelength was 291 nm (0-3 min, vitamin B6),273 nm(3-5 min, hydrochlorothiazide),251 nm(5-11 min, rutin, promethazine hydrochloride) and 218 nm(11 min, reserpine), column temperature was 30 ℃, and injection volume was 20 μl. RESULTS: The linear range was 84.0-1 344.0 μg/ml for vitamin B6(r=0.999 8),171.0-2 736.0 μg/ml for hydrochlorothiazide(r=0.999 2),428.0-6 848.0 μg/ml for rutin(r=0.999 9),173.5-2 776.0 μg/ml for promethazine hydrochloride(r=0.999 5) and 5.03-80.48 μg/ml for reserpine(r=0.999 1); limits of quantification were 18.80,14.92, 25.70, 12.20, 4.99 ng, limits of detection were 5.54, 4.50, 8.04, 3.66 1.50 ng, respectively; RSDs of precision, stability and reproducibility tests were lower than 1.0%; recoveries were 98.20%-100.60%(RSD=0.89%,n=6),99.11%-100.28%(RSD=0.39%,n=6), 99.07%-100.12%(RSD=0.38%,n=6), 98.27%-99.70%(RSD=0.50%,n=6), 97.41%-99.40%(RSD=0.88%,n=6), respectively. CONCLUSIONS: The method is simple, accurate with high sensitivity and good reproducibility, and can be used for the simultaneous determination of vitamin B6, hydrochlorothiazide, rutin, promethazine hydrochloride and reserpine in Compound trizin and tutinum tablet.
期刊: 2016年第27卷第33期
作者: 范桂强,宋更申,杜艳雪,张李莉
AUTHORS: FAN Guiqiang,SONG Gengshen,DU Yanxue,ZHANG Lili
关键字: 高效液相色谱法;复方三嗪芦丁片;维生素B6;氢氯噻嗪;芦丁;盐酸异丙嗪;利血平;含量测定
KEYWORDS: HPLC; Compound trizin and tutinum tablet; Vitamin B6; Hydrochlorothiazide; Rutin; Promethazine hydrochloride; Reserpine; Content determination
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