HPLC法同时测定不同产地羊蹄药材中大黄素、大黄酚、大黄素甲醚的含量
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篇名: HPLC法同时测定不同产地羊蹄药材中大黄素、大黄酚、大黄素甲醚的含量
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摘要: 目的:建立同时测定羊蹄药材中大黄素、大黄酚、大黄素甲醚含量的方法,并比较不同产地药材各成分含量差异。方法:采用高效液相色谱法。色谱柱为Agilent C18,流动相为甲醇-0.1%磷酸溶液(梯度洗脱),流速为1.0 ml/min,检测波长为254 nm,柱温为35 ℃,进样量为20 μl。结果:大黄素、大黄酚、大黄素甲醚检测质量浓度线性范围分别为2.04~24.48 μg/ml(r=0.999 7)、2.00~24.00 μg/ml(r=0.999 5)、1.02~12.24 μg/ml(r=0.999 8);精密度、稳定性、重复性试验的RSD<2.0%;加样回收率分别为97.09%~102.65%(RSD=1.79%,n=9)、97.10%~100.31%(RSD=0.97%,n=9)、95.45%~100.18%(RSD=1.45%,n=9)。结论:该方法检测简便,精密度、稳定性、重复性好,可用于羊蹄药材中大黄素、大黄酚、大黄素甲醚含量的同时测定;不同产地药材中各成分含量差异显著。
ABSTRACT: OBJECTIVE: To establish a method for contents determination of emodin, chrysophanol and physcion in Rumex japonicus,and compared the differences of components in medicinal materials from different producing areas. METHODS: HPLC was performed on the column of Agilent C18 with mobile phase of methanal-0.1% phosphoric acid solution (gradient elution) at a flow rate of 1.0 ml/min, detection wavelength was 254 nm, column temperature was 35 ℃,and injection volume was 20 μl. RESULTS: The linear range was 2.04-24.48 μg/ml for emodin (r=0.999 7), 2.00-24.00 μg/ml for chrysophanol (r=0.999 5) and 1.02-12.24 μg/ml for physcion (r=0.999 8); RSDs of precision, stability and reproducibility tests were lower than 2.0%; recoveries were 97.09%-102.65%(RSD=1.79%,n=9), 97.10%-100.31% (RSD=0.97%,n=9) and 95.45%-100.18%(RSD=1.45%, n=9). CONCLUSIONS: The method is simple with high precision, stability and reproducibility, and can be used for the simultaneous determination of emodin, chrysophanol and physcion in R. japonicas.There are obvious differences in medicinal materials from different producing areas.
期刊: 2016年第27卷第33期
作者: 何盛江,聂阳,陈刚,焦豪妍,杨燕军
AUTHORS: HE Shengjiang,NIE Yang,CHEN Gang,JIAO Haoyan,YANG Yanjun
关键字: 高效液相色谱法;羊蹄;大黄素;大黄酚;大黄素甲醚;含量测定
KEYWORDS: HPLC; Rumex japonicus; Emodin; Chrysophanol; Physcion; Content determination
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