HS-SPME-GC-MS法快速检测积雪草挥发性成分
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篇名: HS-SPME-GC-MS法快速检测积雪草挥发性成分
TITLE:
摘要: 目的:建立快速检测积雪草挥发性成分的方法。方法:采用顶空固相微萃取-气相色谱-质谱联用法(HS-SPME-GC-MS)。色谱条件:色谱柱为HP-5 MS石英弹性毛细管柱,载气为高纯氦气(99. 999%),流速为1.0 ml/min,进样口温度为250 ℃,程序升温,分流进样,分流比为10:1。质谱条件:电离方式为电子轰击离子源,电离能量为80 eV,离子源温度为230 ℃,四极杆温度为150 ℃,传输线温度为280 ℃,电子倍增器电压为1 494 V,扫描范围为m/z 30~400。以面积归一化法测定各挥发性成分的相对含量。结果:从积雪草中鉴定出16个挥发性成分,占总峰面积的79.22%,其主要挥发性成分为(E)-β-金合欢烯 (36.97%)、石竹烯 (13.61%)、大根香叶烯 D (10.95%)、α-菖蒲二烯 (2.76%) 和香树烯 (2.33%)。结论:该研究基本明确了积雪草的主要挥发性成分,为进一步认识积雪草提供了一定科学依据。
ABSTRACT: OBJECTIVE: To establish a method for the rapid detection of the volatile constituents in Centella asiatica. METHODS: HS-SPME-GC/MS was adopted, the chromatographic conditions: column was HP-5 MS fused silica capillary column, carrier gas was high-purity helium (99.999%) at a flow rate of 1.0 ml/min, inlet temperature was 250 ℃ (temperature programmed), split injection, split ratio was 10 ∶ 1. MS conditions: ionization was electrospray ionization source, ionization energy was 80 eV, ion source temperature was 230 ℃, quadrupole temperature was 150 ℃, the transmission line temperature was 280 ℃, electron multiplier voltage was 1 494 V, and scan range was m/z 30-400. Retention index method was combined with peak area normalization method to determine the relative percentage contents of each volatile constituent. RESULTS: Totally 16 volatile constituents were identified from C. asiatica, accounting for 79.22% to the total peak area, the main volatile constituents were (E) -β- farnesene (36.97%), caryophyllene (13.61%),germacrene D(10.95%), α-acoradiene (2.76%) and (-)-alloaromadendrene (2.33%). CONCLUSIONS: The study basically clears the main volatile constituents of C. asiatica, and provides certain scientific basis.
期刊: 2016年第27卷第33期
作者: 张伟,张娟娟,尹震花,康文艺
AUTHORS: ZHANG?Wei,ZHANG?Juanjuan,YIN Zhenhua,KANG Wenyi
关键字: 积雪草;挥发性成分;快速检测;顶空固相微萃取;气相色谱-质谱联用法
KEYWORDS: Centella?asiatica; Volatile?constituent; Rapid?detection; HS-SPME;?GC-MS
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