石柑子药材的HPLC指纹图谱研究
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篇名: 石柑子药材的HPLC指纹图谱研究
TITLE:
摘要: 目的:建立石柑子药材的高效液相色谱(HPLC)指纹图谱。方法:采用HPLC法。色谱柱为Thermo Hypersil ODS-2,流动相为甲醇-0.4%磷酸溶液(梯度洗脱),流速为1.0 ml/min,检测波长为300 nm,柱温为30 ℃,进样量为10 μl。以阿魏酰酪胺色谱峰为参照峰,测定24批药材的HPLC图谱,采用《中药色谱指纹图谱相似度评价系统》(2004A版)进行共有峰鉴定和相似度评价。结果:24批石柑子药材有16个共有峰,其中21批药材的相似度>0.8。结论:该研究所建指纹图谱专属性强、结果稳定,可为石柑子药材的质量控制提供依据。
ABSTRACT: OBJECTIVE: To establish the HPLC fingerprint of Pothos chinensis. METHODS: HPLC was performed on the column of Thermo Hypersil ODS-2 with mobile phase of methanol-0.4% phosphoric acid solution (gradient elution) at a flow rate of 1.0 ml/min, detection wavelength was 300 nm, column temperature was 30 ℃,and injection volume was 10 μl. Using ferulic acid tyramine peak as reference, HPLC spectrum of 24 batches of medicinal herbs was determined, and TCM Chromatographic Fingerprint Similarity Evaluation System(2004 edition)was adopted for common peak identification and similarity evaluation. RESULTS: There were 16 common peaks totally in the 24 batches ,similarity of the 21 batches of medicinal materials were higher than 0.8. CONCLUSIONS: The established fingerprint is specific, stable, and can provide basis for quality control of P. chinensis.
期刊: 2016年第27卷第33期
作者: 罗文汇,刘布鸣,彭丽诗,邱宏聪,陈昭,江洁怡
AUTHORS: LUO Wenhui,LIU Buming,PENG Lishi,QIU Hongcong,CHEN Zhao,JIANG Jieyi
关键字: 石柑子;高效液相色谱法;指纹图谱
KEYWORDS: Pothos chinensis; HPLC; Fingerprint
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