多指标正交试验优化女贞子中环烯醚萜苷的提取工艺
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篇名: 多指标正交试验优化女贞子中环烯醚萜苷的提取工艺
TITLE:
摘要: 目的:优化女贞子中环烯醚萜苷成分的提取工艺。方法:以特女贞苷、橄榄苦苷和女贞苷G13的含量为考察指标,以乙醇体积分数、溶剂倍量、提取时间和提取次数为考察因素,采用正交试验对女贞子中环烯醚萜苷类成分的提取工艺进行优化并进行验证试验。结果:4种因素对女贞子环烯醚萜苷类成分的提取量均有显著影响,并以提取次数的影响最为显著;最优提取工艺为14倍量70%乙醇回流提取3次,每次2.5 h;验证试验结果中环烯醚萜苷总含量平均值为83.58 mg/g(RSD=0.94%,n=3)。结论:优化的提取工艺稳定、合理,可用于女贞子中环烯醚萜苷类成分的提取。
ABSTRACT: OBJECTIVE: To optimize the extraction process for iridoid glycosides from Ligustrum lucidum. METHODS: Using the contents of specnuezhenide, oleuropein and nuezhenoside G13 as parameters, the extraction technology of iridoid glycosides from L. lucidum was optimized by orthogonal test with the concentration of ethanol, solvent volume, extraction time and extraction times as factors. The verification test was conducted. RESULTS: 4 factors significantly affected the extraction amount of iridoid glycosides from L. lucidum, with the most prominent effect exerted by extraction times. The optimal extraction process was refluxing 3 times with 14-fold 70% ethanol, 2.5 h each time. The validation test showed that the average total content of iridoid glycosides from L. lucidum was 83.58 mg/g (RSD=0.94%, n=3). CONCLUSIONS: The optimal extraction process is stable and reasonable, and can be used for the extraction of iridoid glycosides from L. lucidum.
期刊: 2016年第27卷第28期
作者: 杨阳,易骏,马利,吴建国,吴锦忠,吴岩斌
AUTHORS: YANG Yang,YI Jun,MA Li,WU Jianguo,WU Jinzhong,WU Yanbin
关键字: 女贞子;环烯醚萜苷;正交试验;提取工艺;特女贞苷;橄榄苦苷;女贞苷G13
KEYWORDS: Ligustrum lucidum; Iridoid glycosides; Orthogonal test; Extraction process; Specnuezhenide; Oleuropein; Nuezhenoside G13
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