人参醇提后药渣中人参酸性多糖的分离纯化工艺研究
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篇名: 人参醇提后药渣中人参酸性多糖的分离纯化工艺研究
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摘要: 目的:以人参醇提后的药渣制备的喷雾粉为原料,优化人参酸性多糖的分离、纯化等工艺。方法: 采用以D-半乳糖醛酸为对照品计的人参酸性多糖量等为指标,优化树脂分离纯化、脱盐、干燥等制备工艺中的方法及条件,并进行小试和中试工艺验证。结果:采用D900型阴离子交换树脂分离纯化人参酸性多糖,上样量为7.2 mg/g(多糖粗品/干树脂),径高比为1 ∶ 7;先用水再用0.3 mol/L的NaCl溶液以2倍柱体积(BV)/h的流速洗脱5 BV;超滤法脱盐,截留分子质量为1 kD;选择喷雾干燥方法制备人参酸性多糖粉末。小试验证试验中产品纯度为94.7%,中试验证试验中各指标的RSD≤2.42%(n=3)。结论 :优化所得人参酸性多糖的分离、纯化工艺稳定可靠、简便易行,适合于工业化生产,且产品纯度较高。
ABSTRACT: OBJECTIVE: To optimize the separation and purification technology of ginseng acidic polysaccharides, with the spray powder made of the residue after ethanol extraction from Panax ginseng as the raw material. METHODS: Taking the content of ginseng acidic polysaccharides, which was calculated with D-galacturonic acid as the reference substance, as the index, the method and condition in the preparation technology including resin separation and purification, desalination and drying were optimized, and the lab-scale test and pilot-scale test were conducted for verification. RESULTS: The D900 anion resin was used to purify and separate ginseng acidic polysaccharides, where elution was made successively with water and 0.3 mol/L NaCl solution at a flow rate of 2 BV/h, with a sample amount of 7.2 mg/g (crude polysaccharide/dry resin), a diameter to height ratio of 1 ∶ 7 and an elution volume of 5 BV. The ultrafiltration method was used for desalination, where molecular weight cut-off was 1 kD. Spray drying was chosen for the preparation of ginseng acidic polysaccharides powder, the purity of which was 94.7% in the lab-scale test. In the pilot-scale test, the RSDs of all indexes were lower than or equal to 2.42% (n=3). CONCLUSIONS: The optimized separation and purification technology of ginseng acidic polysaccharides is stable, reliable, simple, easy, and it is suitable for industrialized production of high-purity products.
期刊: 2016年第27卷第28期
作者: 王贵金,杜红娜,乔莉,毕丹,贾继明
AUTHORS: WANG Guijin,DU Hongna,QIAO Li,BI Dan,JIA Jiming
关键字: 人参;酸性多糖;D-半乳糖醛酸;分离;纯化;制备工艺
KEYWORDS: Panax ginseng; Acidic polysaccharides; D-galacturonic acid; Separation; Purification; Preparation technology
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