清胰解毒方对ERCP术后胰腺炎模型大鼠的改善作用
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篇名: 清胰解毒方对ERCP术后胰腺炎模型大鼠的改善作用
TITLE:
摘要: 目的:考察清胰解毒方对内镜逆行胰胆管造影术(ERCP)后胰腺炎大鼠的改善作用。方法:将60只大鼠随机分为正常组、模型组、阳性对照组(奥曲肽注射液50 μg/kg,sc)和清胰解毒方高、中、低剂量组[56.5、33.9、11.3 g(生药)/kg,ig],每组10只。除正常组外,其余各组大鼠复制ERCP术后胰腺炎模型。成模后,各给药组大鼠给予相应药物,正常组和模型组大鼠ig蒸馏水。测定大鼠血清中白细胞介素10(IL-10)、肿瘤坏死因子α(TNF-α)、胃动素和淀粉酶水平;观察胰腺组织病理变化,并进行评分。结果:与正常组比较,模型组和各给药组大鼠血清中淀粉酶、TNF-α水平显著升高,胃动素、IL-10水平显著降低(P<0.05);胰腺组织发生明显炎性病变,病理评分显著升高(P<0.05);与模型组比较,各给药组大鼠上述指标均得到显著改善,且清胰解毒方各剂量组改善程度优于阳性对照组,差异均有统计学意义(P<0.05)。结论:清胰解毒方对大鼠ERCP术后胰腺炎有一定的改善作用,能够降低血清中淀粉酶、TNF-α水平,升高血清中胃动素和IL-10水平。
ABSTRACT: OBJECTIVE: To investigate the effects of Qingyi jiedu decoction on pancreatitis rats after endoscopic retrograde cholangiopancreatography (ERCP). METHODS: 60 rats were randomly divided into normal group, model group, positive control group (Octreotide injection 50 μg/kg, sc) and Qingyi jiedu decoction high-dose, medium-dose and low-dose groups [56.5, 33.9, 11.3 g(crude drug)/kg,ig] with 10 rats in each group. Except for normal group, post-ERCP pancreatitis model was induced in those groups. After modeling, treatment groups were given relevant medicine, and normal group and model group were given distilled water intragastrically. The serum levels of IL-10, TNF-α, motilin and amylase were determined. The pathological changes of pancreatic tissues were observed and scored. RESULTS: Compared with normal group, the serum levels of amylase and TNF-α in model group and treatment groups were increased significantly, while the levels of motilin and IL-10 were significantly decreased (P<0.05); obvious inflammation was found in pancreatic tissues, and pathological score increased significantly (P<0.05). Compared with model group, above indexes of treatment groups were improved significantly, and Qingyi jiedu decoction groups were better than positive control group, with statistical significance (P<0.05). CONCLUSIONS: Qingyi jiedu decoction can improve pancreatitis after ERCP of rats, reduce the serum levels of amylase and TNF-α, and increase the serum levels of motilin and IL-10.
期刊: 2016年第27卷第28期
作者: 余金钟,党中勤,张勤生,李波,党志博
AUTHORS: YU Jinzhong,DANG Zhongqin,ZHANG Qinsheng,LI Bo,DANG Zhibo
关键字: 清胰解毒方;胰腺炎;内镜逆行胰胆管造影术;大鼠
KEYWORDS: Qingyi jiedu decoction; Pancreatitis; Endoscopic retrograde cholangiopancreatography; Rats
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