HPLC法同时测定野老鹳草中5种活性成分的含量
x

请在关注微信后,向客服人员索取文件

篇名: HPLC法同时测定野老鹳草中5种活性成分的含量
TITLE:
摘要: 目的:建立同时测定野老鹳草中没食子酸、原儿茶酸、儿茶素、柯里拉京、短叶苏木酚含量的方法。方法:采用高效液相色谱法。色谱柱为 WondaSil C18 WR,流动相为乙腈-0.1%磷酸溶液(梯度洗脱),流速为1.0 ml/min,检测波长为280 nm,柱温为40 ℃,进样量为20 μl。结果:没食子酸、原儿茶酸、儿茶素、柯里拉京、短叶苏木酚的检测质量浓度线性范围分别为0.02~20.02、0.01~20.10、0.02~19.78、0.02~20.02、0.02~20.10(r=0.999 9、0.999 7、0.999 6、0.999 9、0.999 5);精密度、稳定性、重复性试验的RSD<3.0%;加样回收率分别为95.1%~100.6%(RSD=2.20%,n=6)、95.8%~100.6%(RSD=1.74%,n=6)、95.1%~101.9%(RSD=2.71%,n=6)、97.7%~103.1%(RSD=2.04%,n=6)、95.3%~99.0%(RSD=1.46%,n=6)。结论:该方法操作简便、结果准确、重复性好,可用于同时测定野老鹳草中没食子酸、原儿茶酸、儿茶素、柯里拉京、短叶苏木酚的含量。
ABSTRACT: OBJECTIVE: To establish a method for simultaneous determination of gallic acid, protocatechuic acid, catechuic acid, corilagin and brevifolincarboxylic acid in Geranium carolinianum. METHODS: HPLC was performed on the column of WondaSil C18 WR with mobile phase of acetonitrile-0.1% Phosphoric acid (gradient elution) at a flow rate of 1.0 ml/min, the detection wavelength was 280 nm, the column temperature was 40 ℃, and the volume injection was 20 μl. RESULTS: The linear range was 0.02-20.02 for gallic acid (r=0.999 9), 0.01-20.10 for protocatechuic acid (r=0.999 7),0.02-19.78 for catechuic acid (r=0.999 6),0.02-20.02 for corilagin(r=0.999 9) and 0.02-20.10 for brevifolincarboxylic acid (r=0.999 5); RSDs of precision, stability and reproducibility tests were lower than <3.0%; recoveries were 95.1%-100.6% (RSD=2.20%,n=6), 95.8%-100.6% (RSD=1.74%,n=6), 95.1%-101.9%(RSD=2.71%,n=6), 97.7%-103.1%(RSD=2.04%,n=6) and 95.3%-99.0% (RSD=1.46%,n=6). CONCLUSIONS: The method is simple, accurate and reproducible, and can be used for the contents determination of gallic acid, protocatechuic acid, catechuic acid, corilagin and brevifolincarboxylic acid in G. carolinianum.
期刊: 2016年第27卷第21期
作者: 王如意,刘纲勇,梁晓欣,廖丽嫦
AUTHORS: WANG Ruyi,LIU Gangyong,LIANG Xiaoxin,LIAO Lichang
关键字: 野老鹳草;没食子酸;原儿茶酸;儿茶素;柯里拉京;短叶苏木酚;高效液相色谱法
KEYWORDS: Geranium carolinianum; Gallic acid; Protocatechuic acid; Catechuic acid; Corilagin; Brevifolincarboxylic acid; HPLC
阅读数: 206 次
本月下载数: 5 次

* 注:未经本站明确许可,任何网站不得非法盗链资源下载连接及抄袭本站原创内容资源!在此感谢您的支持与合作!