黔产荞麦黄酮类成分的含量测定与分析
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篇名: 黔产荞麦黄酮类成分的含量测定与分析
TITLE:
摘要: 目的:建立测定荞麦中芦丁、槲皮素和总黄酮含量的方法,并比较不同品种及产地间荞麦中黄酮类成分的含量差异。方法:采用高效液相色谱法测定药材中芦丁和槲皮素含量:色谱柱为Agilent C18,流动相为甲醇-0.4%磷酸(梯度洗脱),流速为1 ml/min,检测波长为360 nm,柱温为 40 ℃,进样量为10 μl;采用紫外分光光度法测定药材中总黄酮含量:参比溶液为甲醇,检测波长为360 nm,对照品为芦丁。采用统计学方法分析同一产地苦荞、甜荞及不同产地苦荞的黄酮类成分差异。结果:芦丁、槲皮素检测进样量线性范围分别为0.400 9~16.03、0.009 9~0.396 0 μg(r=0.999 4、0.999 7);精密度、稳定性、重复性试验的RSD<2%;加样回收率分别为99.33%~104.00%(RSD=1.32%,n=9)、96.92%~101.66%(RSD=1.60%,n=9)。总黄酮(以芦丁计)检测质量浓度线性范围为4.14~41.4 mg/L(r=0.999 9);精密度、稳定性、重复性试验的RSD<2%;加样回收率为96.07%~101.96%(RSD=2.63%,n=9)。同一地区苦荞黄酮类成分含量明显高于甜荞,毕节地区苦荞该类成分含量高于其他产地。结论:该方法操作简便、稳定、重复性好,可用于荞麦中芦丁、槲皮素和总黄酮含量的测定。本研究可为荞麦资源开发利用提供理论依据。
ABSTRACT: OBJECTIVE: To establish a method for contents determination of rutin, quercetin and total flavonoids in Buckwheat, and compare the difference of flavone in buckwheat in different varieties and areas. METHODS: HPLC was adopted for content determination of rutin and quercetin: the column was Aglient C18 with mobile phase of methanol-0.4% phosphoric acid (gradient elution) at a flow rate of 1 ml/min, detection wavelength was 360 nm, column temperature was 40 ℃, and injection volume was 10 μl; UV spectrophotometry was adopted for content determination of total flavonoids: reference solution was methanol, detection wavelength was 360 nm, the standard was rutin. Statistical method was used to analyze the differences among the Fagopyrum tataricum, F. esculentum and flavonoids of F. tataricum from different areas. RESULTS: The linear range was 0.400 9-16.03 μg for rutin (r=0.999 4) and 0.009 9-0.396 0 μg for quercetin (r=0.999 7), RSDs of precision, stability and reproducibility tests were lower than 2%, recoveries were 99.33%-104.00% (RSD=1.32%,n=9) and 96.92%-101.66% (RSD=1.60%,n=9), respectively. The linear range of total flavonoids (recovded by rutin) was 4.14-41.4 mg/L (r=0.999 9), RSDs of precision, stability and reproducibility tests were lower than 2, and recovery was 96.07%-101.96%(RSD=2.63%,n=9). The content of flavonoids on F. esculentum is significantly higher than in F. tataricum from the same area, and F. tataricum in Bijie area is better than other places. CONCLUSIONS: The method is simple, stable, reproducible, and can be used for the contents determination of rutin, quercetin and total flavonoids in F. esculentum. The study can provide theoretical basis for developing and using buckwheat.
期刊: 2016年第27卷第21期
作者: 迟明艳,李光芳,刘丽娜
AUTHORS: CHI Mingyan,LI Guangfang,LIU Li’na
关键字: 贵州;苦荞;甜荞;黄酮类成分
KEYWORDS: Guizhou province; Fagopyrum esculentum; Fagopyrum tataricum; Flavonoids
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