HS-GC-MS法分析江西产薄荷不同部位的挥发性成分
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篇名: HS-GC-MS法分析江西产薄荷不同部位的挥发性成分
TITLE:
摘要: 目的:建立分析薄荷不同部位(根、茎、叶)挥发性成分的方法。方法:采用顶空气相色谱-质谱联用法(HS-GC-MS)。GC条件:HP-5MS石英毛细管柱,载气为He(恒流模式),柱流速为1.0 ml/min,进样量为1.0 μl,进样口模式为分流模式,分流比为10 ∶ 1,进样口温度为250 ℃,程序升温。MS条件:电离源为电子轰击离子源,电离能为70 eV,电子倍增管电压为1 905 V,离子源温度为230 ℃,四极杆温度为150 ℃ ,质量扫描范围为m/z 30~550。结果:从江西产薄荷根、茎、叶分别鉴定出10、14和48种挥发性成分,分别占各部位挥发油总量的90.03%、92.79%和95.93%。三者共鉴定出51个挥发性成分,其中有7个共有化学成分,且均以氧化胡椒酮为主要成分。结论:该方法操作简便、稳定、重复性好,可用于江西产薄荷不同部位的挥发性成分分析。江西产薄荷不同部位所含挥发性成分差异较大,其中以薄荷叶中的挥发性成分最多。
ABSTRACT: OBJECTIVE: To establish a method for analyzing the volatile constituents of different parts(root, stem and leaf) of Mentha haplocalyx. METHODS: HS-GC-MS was performed. GC conditions: the column was HP-5MS capillary quartz column, carrier gas was He (constant current mode), flow rate was 1.0 ml/min, volume injection was 1.0 μl, inlet was split mode with split ratio of 10 ∶ 1, temperature was 250 ℃ (temperature programmed). MS conditions: ionization source was electron bombardment, energy was 1 905 V, temperature was 230 ℃, quadrupole temperature was 150 ℃, and the scanning range was m/z 30-550. RESULTS: Totally 10,14 and 48 volatile components were identified from the root, stem and leaf, which accounted for 90.03%, 92.79% and 95.93% of the capacity of the total essential oil from each part of it. And totally 51 volatile compounds was identified, including 7 kinds that shared the same chemical components that regard the piperitone oxide as the main ingredient. CONCLUSIONS: The method is simple, stable and reproducible, and can be used for the analysis of volatile components of different parts of M. haplocalyx. The volatile components in different parts are quite different, and most is in the leaf.
期刊: 2016年第27卷第21期
作者: 吴司琪,伍振峰,张小飞
AUTHORS: WU Siqi,WU Zhenfeng,ZHANG Xiaofei
关键字: 顶空进样;气相色谱-质谱联用法;薄荷;挥发性成分;不同部位
KEYWORDS: Headspace sampling; Gas chromatography mass spectrometry; Mentha haplocalyx; Volatile composition; Different parts
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