白脉软膏对家兔坐骨神经损伤的保护作用
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篇名: 白脉软膏对家兔坐骨神经损伤的保护作用
TITLE:
摘要: 目的:研究白脉软膏对家兔坐骨神经损伤的保护作用。方法:采用钳夹法复制家兔坐骨神经损伤模型。180只家兔随机分为6组,每组30只(其中7、14、28 d各取10只),分别为假手术组(外敷白脉软膏空白基质)、模型组(外敷白脉软膏空白基质)、阳性组[ig甲钴胺片溶液1.25×10-4 g/(kg·d)]和白脉软膏低、中、高剂量组[外敷白脉软膏0.33、0.67、1.34 g/(kg·d)],每天1次,连续用药7、14、28 d。使用步态仪检测家兔患足着地力;切取坐骨神经进行病理学检查;免疫组化法检测神经组织中一氧化氮合酶(NOS)和N-甲基-D-天门冬氨酸(NMDA)水平。结果:白脉软膏可显著减轻坐骨神经病理性损伤,减轻水肿与炎症反应的程度;给药后7、14、28 d,与模型组比较,各给药组兔患足着地力明显下降(P<0.01);给药后14、28 d,阳性组与白脉软膏各剂量组家兔患足着地力均显著提高,神经组织中NOS、NMDA水平均显著降低(P<0.05)。结论:白脉软膏对家兔功能损伤的坐骨神经具有明显保护作用。
ABSTRACT: OBJECTIVE: To study the protective effects of Baimai ointment on sciatic nerve injury in rabbit. METHODS: The rabbit sciatic nerve injury model was induced by forceps operation. 180 rabbits were randomly divided into 6 groups, with 30 rabbits in each group (10 rabbits of 7, 14, 28 d), including sham operation group (blank matrix of Baimai ointment for external use), model group (same as sham operation group), positive group [ig Mecobalamine tablet solution 1.25×10-4 g/(kg·d)] and Baimai low-dose, medium-dose and high-dose groups [Baimai ointment 0.33, 0.67 and 1.34 g/(kg·d) for external use], once a day, for consecutive 7, 14 and 28 d. Gait instrument was used to test the  foot-touch-land force of rabbit; pathology examination was conducted for sciatic nerve resection; immunohistochemistry was used to detect the levels of NOS and NMDA in nerve tissue. RESULTS: Baimai ointment can significantly relieve sciatic nerve injury, edema and inflammatory reaction; 7, 14 and 28 d after medication, compared with model group, foot-touch-land force decreased significantly in rabbits with sciatic nerve injury (P<0.01); 14, 28 d after medication, foot-touch-land force of rabbits increased significantly in positive group, Baimai ointment groups, while the levels of NOS and NMDA decreased in nervous tissue (P<0.05). CONCLUSIONS: Baimai ointment can protect sciatic nerve with function injury in rabbits.
期刊: 2016年第27卷第19期
作者: 路杰,张万强,杨伟鹏,张坚,李富银
AUTHORS: LU Jie,ZHANG Wanqiang,YANG Weipeng,ZHANG Jian,LI Fuyin
关键字: 白脉软膏;坐骨神经损伤;行为学;病理学;一氧化氮合酶;N-甲基-D-天门冬氨酸;家兔
KEYWORDS: Baimai ointment; Sciatic nerve injury; Behavior; Pathology; NOS; NMDA; Rabbit
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