比较雷奈酸锶与甲状旁腺激素(1-34)对卵巢切除大鼠骨质量的影响
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篇名: 比较雷奈酸锶与甲状旁腺激素(1-34)对卵巢切除大鼠骨质量的影响
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摘要: 目的:比较雷奈酸锶与甲状旁腺激素(1-34)对卵巢切除大鼠骨质量的影响。方法:80只SD大鼠,分别接受假手术(A组,n=10)和双侧卵巢切除(B组,n=70)手术,术后3个月B组大鼠随机分为7组,每组10只。分别sc生理盐水[0.9 g/(kg·d),B0组];ig雷奈酸锶低、中、高剂量[0.45、0.9、1.35g/(kg·d),B1~B3组];sc甲状旁腺激素(1-34)低、中、高剂量[30、60、90 μg/(kg·d),连续给药5 d后停止给药2 d,B4~B6组],A组大鼠给药方案同B0组。8周后处死所有大鼠,酶联免疫吸附法(ELISA)检测大鼠血清1型前胶原氨基末端肽(P1NP)、1型胶原C端肽(CTX-1)含量;骨密度仪检测第4腰椎骨密度;微电子计算机断层扫描(CT)检测松质骨相对体积(BV/TV)、 骨小梁厚度 (Tb.Th)、骨小梁数量(Tb.N)和骨小梁分离度(Tb.Sp);压缩试验检测第5腰椎最大载荷和弹性模量。结果:与A组比较,B0~B6组大鼠血清P1NP、CTX-1水平显著升高,第4腰椎骨密度、第5腰椎最大载荷、弹性模量显著降低(P<0.05);B0组大鼠第4腰椎BV/TV水平显著降低、Tb.Sp水平显著升高(P<0.05)。与B0组比较,B1~B3组大鼠第4腰椎骨密度、第5腰椎最大载荷、弹性模量显著升高(P<0.05);B4~B6组大鼠P1NP含量、BV/TV、Tb.N水平、第4腰椎骨密度、第5腰椎最大载荷、弹性模量显著升高,且高于B1~B3组(P<0.05);B1~B6组大鼠Tb.Sp水平显著降低,且B4~B6组低于B1~B3组(P<0.05)。8组大鼠Tb.Th水平比较,B0~B6组大鼠CTX-1含量比较,差异均无统计学意义(P>0.05)。结论:甲状旁腺激素(1-34)抑制去卵巢大鼠骨丢失、改善其椎体骨微观结构和生物力学性能的作用强于雷奈酸锶。
ABSTRACT: OBJECTIVE: To compare the effects of strontium ranelate and PTH (1-34) on bone quality of ovariectomized rats. METHODS: 80 SD rats were randomly divided into sham operation group (group A, n=10) and dual ovariectomy (group B, n=70). 3 months after operation, group B were randomly divided into 7 groups, with 10 rats in each group. B0 group were given normal saline [0.9 g/(kg·d)] subcutaneously; B1-B3 groups were given low-dose, medium-dose and high-dose of strontium ranelate [0.45, 0.9, 1.35 g/(kg·d)] intragastrically; B4-B6 groups were given low-dose, medium-dose and high-dose of PTH (1-34) [30, 60, 90 μg/(kg·d), treated for 5 days, rested for 2 days] subcutaneously. Group A was same to group B0 in therapy regimen. All rats were sacrificed 8 weeks later. The contents of P1NP and CTX-1 in serum of rats were determined by ELISA assay; bone density of 4th lumbar vertebrae was detected by bone densitometer; BV/TV, Tb.Th, Tb.N and Tb.Sp were detected by CT; maximal load and elastic modulus of 5th lumbar vertebrae were measured by compression test. RESULTS: Compared with group A, the serum levels of P1NP and CTX-1 in B0-B6 groups increased significantly, while bone density of 4th lumbar vertebrae, maximal load and elastic modulus of 5th lumbar vertebrae decreased significantly in B0-B3 groups (P<0.05); BV/TV level of 4th lumbar vertebrae decreased significantly, while Tb.Sp level increased significantly in B0 group (P<0.05). Compared with B0 group, bone density of 4th lumbar vertebrae, maximal load and elastic modulus of 5th lumbar vertebrae increased significantly in B1-B3 groups (P<0.05); P1NP content, BV/TV, Tb.N level, bone density of 4th lumbar vertebrae, maximal load and elastic modulus of 5th lumbar vertebrae increased significantly in B4-B6 groups, and were higher than in B1-B3 groups (P<0.05). Tb.Sp level of B1-B6 groups decreased significantly and were lower than those of B1-B3 groups (P<0.05). There was no statistical significance in Tb.Th level among 8 groups and CTX-1 content among B0-B6 groups (P>0.05). CONCLUSIONS: PTH(1-34) is better than strontium ranelate in inhibiting bone loss, improving vertebral bone micro-structure and biomechanical properties of ovariectomized rats.
期刊: 2016年第27卷第19期
作者: 冯云波,刘小坡,曹国龙,张沛,田发明
AUTHORS: FENG Yunbo,LIU Xiaopo,CAO Guolong,ZHANG Pei,TIAN Faming
关键字: 卵巢切除;骨质疏松;雷奈酸锶;甲状旁腺激素(1-34);骨密度;大鼠
KEYWORDS: Ovariectomy; Osteoporosis; Strontium Ranelate; PTH(1-34); BMD; Rat
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