虎杖苷对大鼠肾纤维化的改善作用及其机制研究
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篇名: 虎杖苷对大鼠肾纤维化的改善作用及其机制研究
TITLE:
摘要: 目的:研究虎杖苷对大鼠肾纤维化的改善作用及其机制。方法:将50只大鼠随机分为假手术组、模型组、阳性组(贝那普利,5 mg/kg)和虎杖苷高、低剂量组(100、50 mg/kg),每组10只,除假手术组外,其余各组大鼠均采用单侧输尿管结扎法复制肾纤维化模型。造模成功后,各给药组大鼠ig相应药物,假手术组和模型组大鼠ig 0.5%羧甲基纤维素钠溶液,每天1次,连续4周。观察肾脏组织病理变化并进行评分,检测24 h尿蛋白和血清中尿素氮、肌酐水平,检测肾脏组织中羟脯氨酸含量以及转化生长因子β1(TGF-β1)、纤连蛋白(FN) mRNA的表达。结果:与假手术组比较,模型组大鼠病理评分,24 h尿蛋白,血清中尿素氮、肌酐水平,肾组织中羟脯氨酸含量以及TGF-β1、FN mRNA表达水平均明显升高(P<0.01)。与模型组比较,各给药组大鼠24 h尿蛋白,血清中尿素氮、肌酐以及肾组织中FN mRNA表达水平均明显降低;阳性组和虎杖苷高剂量组大鼠病理评分,肾组织中羟脯氨酸含量以及肾组织中TGF-β1 mRNA表达水平均明显降低(P<0.05或P<0.01)。结论:虎杖苷能够改善单侧输尿管结扎致大鼠肾纤维化,其机制可能与其下调肾脏组织中TGF-β1、FN mRNA表达有关。
ABSTRACT: OBJECTIVE: To study the improvement effects of polydatin on renal fibrosis in rats and its mechanism. METHODS: 50 rats was were randomly divided into sham operation group, model group, positive group (benazepril, 5 mg/kg) and polydatin high-dose and low-dose groups (100, 50 mg/kg), with 10 rats in each group. Except for sham operation group, renal fibrosis model was induced by unilateral ureter obstruction. After modeling, administration groups were given relevant medicine intragastrically, and sham operation group and model group were given 0.5% sodium carboxymethyl cellulose solution once a day for consecutive 4 weeks. The pathological change of renal tissue was scored. 24 h urinary protein and serum levels of urea nitrogen and creatinine were determined, and the content of hydroxyproline, mRAN expression of TGF-β1 and FN were detected in renal tissue. RESULTS: Compared with sham operation group, pathological score, 24 h urinary protein, serum levels of urea nitrogen and creatinine, the content of hydroxyproline in renal tissue, mRNA expression of TGF-β1 and FN were all increased significantly (P<0.01). Compared with model group, 24 h urinary protein, serum levels of urea nitrogen and creatinine and mRNA expression of FN in renal tissue decreased significantly in administation groups; the pathology scores, the content of hydroxyproline in renal tissue and mRNA expression of TGF-β1 of positive group and polydatin high-dose group were all decreased significantly (P<0.05 or P<0.01). CONCLUSIONS: Polydatin can prevent kidney fibrosis induced by unilateral ureteral obstruction, the mechanism of which may be associated with the mRNA expression down-regulation of TGF-β1 and FN in renal tissue.
期刊: 2016年第27卷第19期
作者: 王松,赵晓玉,陈成群,任清华,齐跃东
AUTHORS: WANG Song,ZHAO Xiaoyu,CHEN Chengqun,REN Qinghua,QI Yuedong
关键字: 单侧输尿管结扎;肾纤维化;转化生长因子β1;纤连蛋白;虎杖苷;大鼠
KEYWORDS: Unilateral urethral obstruction; Renal fibrosis; TGF-β1; FN; Polydatin; Rat
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