丁酸钠对慢性应激模型大鼠空间学习记忆能力的影响
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篇名: 丁酸钠对慢性应激模型大鼠空间学习记忆能力的影响
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摘要: 目的:研究丁酸钠对慢性应激模型大鼠空间学习和记忆能力的影响。方法:将SD大鼠随机分为对照组(生理盐水)、模型组(生理盐水)和丁酸钠低、中、高剂量组(50、100、200 mg/kg),每组14只。模型组和丁酸钠各剂量组大鼠给予21 d电击足底刺激,每日1次,每次2 h,以复制慢性应激模型;在刺激前0.5 h ip相应药物。采用Morris水迷宫试验测试各组大鼠的空间学习记忆能力(以逃避潜伏期为指标,连续测试5 d);Western blot法检测海马组织中乙酰化组蛋白H3(acH3)、acH4、磷酸化环磷酸腺苷(cAMP)反应元件结合蛋白(p-CREB)、脑源性神经营养因子(BDNF)的表达情况。结果:与对照组比较,模型组和丁酸钠各剂量组大鼠在测试第2天开始逃避潜伏期明显延长(P<0.05),acH3、acH4、p-CREB、BDNF表达均降低(P<0.05)。与模型组比较,丁酸钠各剂量组大鼠的逃避潜伏期缩短,其中高剂量组最明显(测试第2天开始差异有统计学意义)(P<0.05);acH3、acH4、p-CREB、BDNF表达均增强(P<0.05)。结论:丁酸钠可改善慢性应激造成的大鼠空间学习记忆能力下降,其机制可能是通过恢复大鼠海马区域的组蛋白乙酰化水平,进而上调认知相关蛋白p-CREB、BDNF的表达来实现。
ABSTRACT: OBJECTIVE: To study the effects of sodium butyrate on spatial learning and memory ability in chronic stress model rats. METHODS: SD rats were randomly divided into control group (normal saline), model group (normal saline) and sodium butyrate low-dose, medium-dose and high-dose groups (50, 100, 200 mg/kg) with 14 rats in each group. Model group and sodium butyrate groups received plantar stimulation by electric shock for 21 d, once a day, 2 h each time, to induce chronic stress model.They were given relevant medicine ip 0.5 h before stimulation. Morris water maze test was adopted to test spatial learning and memory ability of rats (using escape latency as index, for consecutive 5 d). Western blot assay was used to detect the expression of acH3, acH4, cAMP, p-CREB and BDNF in hippocampus. RESULTS: Compared with control group, escape latency of model group and sodium butyrate groups prolonged significantly on the second day (P<0.05), and the expression of acH3, acH4, p-CREB and BDNF decreased (P<0.05). Compared with model group, escape latency of sodium butyrate groups shortened, especially in high-dose group (there was statistical significance on the second day of test) (P<0.05); the expression of acH3, acH4, p-CREB, and BDNF enhanced (P<0.05). CONCLUSIONS: Sodium butyrate can improve chronic stress-induced the decrease of spatial learning and memory ability, the mechanism of which may be recovering histone acetylation level of chronic stress rat hippocampus, and increasing cognitive related proteins such as p-CREB and BDNF.
期刊: 2016年第27卷第16期
作者: 彭静
AUTHORS: PENG Jing
关键字: 丁酸钠;慢性应激;大鼠;学习;记忆;认知相关蛋白
KEYWORDS: Sodium butyrate; Chronic stress; Rat; Learning; Memory; Related protein of cognition
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