苦参碱对人膀胱癌BIU-87细胞增殖的抑制作用及其机制研究Δ
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篇名: 苦参碱对人膀胱癌BIU-87细胞增殖的抑制作用及其机制研究Δ
TITLE:
摘要: 目的:研究苦参碱对人膀胱癌BIU-87细胞增殖的抑制作用及其机制。方法:以0(阴性对照)、0.5、1.0、2.0、4.0 mg/ml苦参碱分别作用于人膀胱癌BIU-87细胞24、48、72 h后, MTT法检测细胞活性并计算细胞增殖抑制率;以0(阴性对照)、0.5、1.0、2.0、4.0 mg/ml苦参碱作用于细胞48 h后,流式细胞术检测细胞周期和凋亡率,Western blot 法检测细胞中生存素、天冬氨酸半胱氨酸蛋白酶(Caspase)-3及Caspase-7蛋白的表达。结果:与阴性对照比较,1.0~4.0 mg/ml苦参碱作用24、48、72 h后均对BIU-87细胞增殖有显著的抑制作用(P<0.05或P<0.01),增殖抑制率呈浓度和时间依赖性升高;作用 48 h后,G0/G1期细胞比例升高、S期及G2/M  期细胞比例降低、凋亡率升高(P<0.05或P<0.01)。0.5~4.0 mg/ml苦参碱作用48 h后,细胞中生存素蛋白表达减弱、Caspase-3及Caspase-7蛋白表达增强(P<0.05或P<0.01)。结论:苦参碱可抑制人膀胱癌BIU-87细胞的增殖、阻滞细胞周期、诱导细胞凋亡,其机制可能与调控细胞中生存素及Caspase-3、Caspase-7的表达有关。
ABSTRACT: OBJECTIVE: To investigate inhibitory effects of matrine on the proliferation of human bladder cancer BIU-87 cells and its mechanism. METHODS: The cell viability was detected by MTT assay and inhibitory rate of cell proliferation was calculated after human bladder cancer BIU-87 cells were treated with 0 (negative control), 0.5, 1.0, 2.0 and 4.0 mg/ml matrine for 24, 48 and 72 h, respectively. After treated with 0 (negative control), 0.5, 1.0, 2.0 and 4.0 mg/ml matrine for 48 h, the cell cycle and apoptotic rate were detected by flow cytometry; the expression of Survivin, Caspase-3 and Caspase-7 protein were detected by Western blot assay. RESULTS: Compared with negative control, the proliferation of BIU-87 cells were significantly inhibited after incubated with 1.0-4.0 mg/ml matrine for 24, 48 and 72 h (P<0.05 or P<0.01), and inhibitory rate of cell proliferation increased in concentration and time-dependant manner; after treated for 48 h, the percentage of G0/G1 phase cells and apoptotic rate increased, while the percentage of cells at S phase and G2/M phase were decreased (P<0.05 or P<0.01); the expression of Caspase-3 and Caspase-7 protein increased, while the expression of survivin protein decreased after incubated with 0.5-4.0 mg/ml matrine for 48 h. CONCLUSIONS: Matrine can inhibit the proliferation of human bladder cancer BIU-87 cells, block the cell cycle and induce apoptosis; its mechanism may be related to the expression regulation of Survivin, Caspase-3 and Caspase-7.
期刊: 2016年第27卷第16期
作者: 姚莉,武兴斌,秦龙
AUTHORS: YAO Li,WU Xingbin,QIN Long
关键字: 苦参碱;人膀胱癌BIU-87细胞;增殖;凋亡
KEYWORDS: Matrine; Human bladder cancer BIU-87 cells; Proliferation; Apoptosis
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