HPLC法同时测定益心饮口服液中6种成分的含量
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篇名: HPLC法同时测定益心饮口服液中6种成分的含量
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摘要: 目的:建立同时测定益心饮口服液中丹参素钠、原儿茶醛、毛蕊异黄酮苷、甘草苷、丹酚酸B和丹酚酸A含量的方法。方法:采用高效液相色谱法。色谱柱为Sepax Sapphire-C18,流动相为乙腈-0.2%甲酸(梯度洗脱),流速为0.8 ml/min,检测波长为283 nm,柱温为25 ℃,进样量为10 μl。结果:丹参素钠、原儿茶醛、毛蕊异黄酮苷、甘草苷、丹酚酸B和丹酚酸A检测质量浓度线性范围为25.00~400.00、2.50~40.00、5.00~80.00、6.25~100.00、18.75~300.00、5.00~80.00 μg/ml(r=0.999 9、0.999 9、0.999 9、0.999 9、0.999 8、0.999 8);精密度、稳定性、重复性试验的RSD<2.0%;加样回收率分别为101.52%~102.24%、96.25%~99.25%、99.80%~101.80%、96.60%~100.00%、96.51%~99.77%、97.09%~102.00%,RSD分别为0.23%、1.06%、0.75%、1.29%、1.20%、1.91%(n=6)。结论:该方法操作简单、准确性高、重复性好,可用于同时测定益心饮口服液中丹参素钠、原儿茶醛、毛蕊异黄酮苷、甘草苷、丹酚酸B和丹酚酸A的含量。
ABSTRACT: OBJECTIVE:To establish a method for contents determination of salvianic acid A sodium, protocatechualdehyde, calycosin-7-glycoside, liquiritin, salvianolic acid B and salvianolic acid A. METHODS: HPLC was performed on the column of Sepax Sapphire-C18 with mobile phase of acetonitrile-0.2% formic acid (gradient elution) at a flow rate of 0.8 ml/min, the detection wavelength was 283 nm,the column temperature was 25 ℃, and the injection volume was 10 μl. RESULTS: The linear range was  25.00-400.00 μg/ml for salvianic acid A sodium(r=0.999 9),2.50-40.00 μg/ml for protocatechualdehyde(r=0.999 9),   5.00-80.00 μg/ml for calycosin-7-glycoside(r=0.999 9),6.25-100.00 μg/ml for liquiritin (r=0.999 9), 18.75-300.00 μg/ml for salvianolic acid B(r=0.999 8) and 5.00-80.00 μg/ml for salvianolic acid A(r=0.999 8); RSDs of precision, stability and reproducibility tests were lower than 2.0%; recoveries were 101.52%-102.24%(RSD=0.23%,n=6),96.25%-99.25%(RSD=1.06%,n=6),99.80%-101.80%(RSD=0.75%,n=6),96.60%-100.00%(RSD=1.29%,n=6),96.51%-99.77%(RSD=1.20%,n=6) and 97.09%-102.00%(RSD=1.91%,n=6),respectively. CONCLUSIONS:The method is simple, accurate and reproducible, and can be used for simultaneous determination of salvianic acid A sodium, protocatechualdehyde, calycosin-7-glycoside, liquiritin, salvianolic acid B and salvianolic acid A in Yixinyin oral solution.
期刊: 2016年第27卷第9期
作者: 陈力,高守红,熊筱娟,朴淑娟,张凤
AUTHORS: CHEN Li,GAO Shouhong,XIONG Xiaojuan,PIAO Shujuan,ZHANG Feng
关键字: 高效液相色谱法;益心饮口服液;丹参素钠;原儿茶醛;毛蕊异黄酮苷;甘草苷;丹酚酸B;丹酚酸A
KEYWORDS: HPLC; Yixinyin oral solution; Salvianic acid A sodium; Protocatechualdehyde; Calycosin-7-glycoside; Liquiritin; Salvianolic acid B; Salvianolic acid A
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