HPLC法同时测定化痰平喘片中黄芩苷、黄芩素和汉黄芩素的含量
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篇名: HPLC法同时测定化痰平喘片中黄芩苷、黄芩素和汉黄芩素的含量
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摘要: 目的:建立同时测定化痰平喘片中黄芩苷、黄芩素和汉黄芩素含量的方法。方法:采用高效液相色谱法。色谱柱为Diamonsil C18,流动相为甲醇-0.1%磷酸(梯度洗脱),流速为1.0 ml/min,检测波长为277 nm,柱温为30 ℃,进样量为10 μl。结果:黄芩苷、黄芩素、汉黄芩素检测进样量线性范围为0.069 04~0.690 4(r=0.999 4)、0.054 56~0.545 6(r=0.999 6)、0.030 36~0.303 6 μg(r=0.999 1);精密度、稳定性、重复性试验的RSD<2%;加样回收率分别为97.64%~99.06%(RSD=0.57%,n=6)、98.31%~100.64%(RSD=0.85%,n=6)、97.53%~99.48%(RSD=0.76%,n=6)。结论:该方法操作简便、稳定、重复性好,可用于化痰平喘片中黄芩苷、黄芩素和汉黄芩素含量的同时测定。
ABSTRACT: OBJECTIVE:To establish a method for the simultaneous determination of baicalin, baicalein and wogonin in Huatan pingchuan tablet. METHODS: HPLC was performed on the column was Diamonsil C18 with mobile phase of methanol -0.1% phosphoric acid(gradient elution) at a flow rate of 1.0 ml/min, detection wavelength was 277 nm, column temperature was 30 ℃,and the injection volume was 10 μl. RESULTS:The linear range was 0.069 04-0.690 4 μg for baicalin(r=0.999 4),0.054 56- 0.5456 μg for baicalein(r=0.999 6) and 0.030 36-0.303 6 μg for wogonin(r=0.999 1); RSDs of precision, stability and reproducibility tests were lower than 2%; recoveries were 97.64%-99.06%(RSD=0.57%,n=6), 98.31%-100.64%(RSD=0.85%,n=6) and 97.53%-99.48%(RSD=0.76%,n=6). CONCLUSIONS: The method is simple, stable and reproducible, and can be used for the contents determination of baicalin, baicalein and wogonin in Huatan pingchuan tablet.
期刊: 2016年第27卷第6期
作者: 陈翔,张红萍,许景景
AUTHORS: CHEN Xiang,ZHANG Hongping,XU Jingjing
关键字: 高效液相色谱法;化痰平喘片;黄芩苷;黄芩素;汉黄芩素
KEYWORDS: HPLC; Huatan pingchuan tablet; Baicalin; Baicalein; Wogonin
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