篇名: HPLC法同时测定心舒乐片中丹酚酸B、葛根素和羟基红花黄色素A的含量
摘要: 目的:建立同时测定心舒乐片中丹酚酸B、葛根素和羟基红花黄色素A含量的方法。方法:采用高效液相色谱法。色谱柱为Welchrom C18,流动相为乙腈-0.1%磷酸(梯度洗脱),流速为1.0 ml/min,检测波长为280 nm,柱温为35 ℃,进样量为10 μl。结果:丹酚酸B、葛根素、羟基红花黄色素A检测质量浓度线性范围分别为31.54~189.22(r=0.999 1)、17.13~102.76(r=0.999 7)、4.27~25.60 μg/ml(r=0.999 5);精密度、稳定性、重复性试验的RSD<2%;加样回收率分别为96.29%~100.88%(RSD=1.73%,n=6)、97.12%~100.57%(RSD=1.15%,n=6)、97.09%~99.95%(RSD=1.01%,n=6)。结论:该方法简便实用、重现性好,专属性强,可有效控制该制剂质量。
ABSTRACT: OBJECTIVE:To establish a method for the simultaneous determination of salvianolic acid B, puerarin and hydroxysafflor yellow A in Xinshule tablet. METHODS: HPLC was performed on a Welchrom C18 with mobile phase of acetonitrile - 0.1% phosphoric acid (gradient elution) at a flow rate of 1.0ml/min, detection wavelength was 280 nm, column temperature was 35 ℃, and injection volume was 10 μl. RESULTS: The linear range was 31.54-189.22 μg/ml for salvianolic acid B (r=0.999 1), 17.13-102.76 μg/ml for puerarin(r=0.999 7) and  4.27-25.60 μg/ml for hydroxysafflor yellow A(r=0.999 5); RSDs of precision, stability and reproducibility tests were lower than 2%; recoveries were 96.29%-100.88% (RSD=1.73%,n=6), 97.12%-100.57% (RSD=1.15%,n=6) and 97.09%-99.95%(RSD=1.01%,n=6), respectively. CONCLUSIONS: The method is simple, practical and reproducible, and can effectively control the quality of preparation.
期刊: 2016年第27卷第6期
作者: 李中娥
关键字: 丹酚酸B;葛根素;羟基红花黄色素A;心舒乐片;高效液相色谱法
KEYWORDS: Salvianolic acid B; Puerarin; Hydroxysafflor yellow A; Xinshule tablet; HPLC
阅读数: 184 次
本月下载数: 4 次

* 注:未经本站明确许可,任何网站不得非法盗链资源下载连接及抄袭本站原创内容资源!在此感谢您的支持与合作!